植物乳桿菌為圓端直桿菌,通常為0.9~1.2vtm×3.0~8.0μm,單個(gè)、成對(duì)或短鏈狀。通常缺乏鞭毛,但能運(yùn)動(dòng)。革蘭氏陽性,不生芽孢。兼性厭氧, 表面菌落直徑約3mm,凸起,呈圓形,表面光滑,細(xì)密,色白,偶爾呈淺黃或深黃色。屬化能異養(yǎng)菌,生長(zhǎng)需要營(yíng)養(yǎng)豐富的培養(yǎng)基,需要泛酸鈣和煙酸,但不需要硫胺素、吡哆醛或吡哆胺、葉酸、維生素B12。能發(fā)酵戊糖或葡萄糖酸鹽,終產(chǎn)物中85%以上是乳酸。通常不還原硝酸鹽,不液化明膠,接觸酶和氧化酶皆陰性。能產(chǎn)生DL一乳酸,有1,6~二磷酸果糖醛縮酶和單磷酸己糖途徑的活性,能在葡萄酸鹽中生長(zhǎng),并產(chǎn)C02。發(fā)酵1分子的核糖或其他的戊糖生成1分子的乳酸和1分子的乙酸。15℃能生長(zhǎng),通常最適生長(zhǎng)溫度為30~35℃。
ATCC菌種名稱: 植物乳桿菌

Lactobacillus plantarum (Orla-Jensen) Bergey et al. 拉丁名
(ATCC? 8014?) 統(tǒng)一編號(hào)
Strain Designations 菌株別名: 17-5 [BUCSAV 217, BUCSAV 449, Glaxo 664, ICPB 2080, NCDO 82, NCIB 6376, NCIB 8014, NCIB 8030] /
Type Strain 模式菌株 : no /
Biosafety Level 生物安全等級(jí) : 1
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Product Format freeze-dried
Storage Conditions 保藏方法
Frozen(冷凍物): -80℃ or colder
Freeze-Dried(凍干物): 2℃ to 8℃
Live Culture(活物): See Propagation Section
Preceptrol? no
Type Strain 模式菌株no
Comments 注釋 Each NCIB culture from a different depositor Accumulation of biotin D-biotin conversion and metabolism Biotin transport and accumulation Formation of acetylmethylcarbinol Effect of pantothenate derivatives on growth and coenzyme-A synthesis Sulfur nutrition Nitrate reduction Effect of oleic acid on free biotin uptake Ribitol-5-phosphate dehydrogenase DNA base composition (45.1 moles % GC)
Medium 培養(yǎng)基 ATCC? Medium 416: Lactobacilli MRS Agar/Broth
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度: 37℃
Atmosphere 需氧情況 : 5% CO2
Name of Depositor 寄存人 E McCoy
References AACC Niacin--microbiological method. St. Paul, MN, 1999 Arch. Biochem. 16: 357-360, 1948.
Costilow RN, Humphreys TW. Nitrate reduction by certain strains of Lactobacillus plantarum. Science 121: 168, 1955. PubMed: 13225760
Lichstein HC, Waller
ATCC?培養(yǎng)基416:乳酸桿菌MRS瓊脂/肉湯
生長(zhǎng)條件溫度:37°C氣氛:5%CO2
傳播程序
1.根據(jù)隨附的說明打開樣品瓶。
2.使用#416肉湯(5至6 mL)的單管,用巴斯德或乙醇提取約0.5至1.0 mL
1.0 mL移液器。 將整個(gè)顆粒再水化。
3.將該等分試樣無菌轉(zhuǎn)移回肉湯管中。 好好混合。
4.使用幾滴懸浮液接種#416瓊脂斜面和/或平板。
5.將管和板在37℃下孵育24小時(shí)。