編號：ATCC 9763
生物體：釀酒酵母NRRL Y-567
產(chǎn)品形式：懸浮在適當(dāng)?shù)睦鋬霰Ｗo(hù)劑中的酵母細(xì)胞有效期
儲存條件：2°C至8°C冷凍干燥培養(yǎng)，低濕度; - 冷凍培養(yǎng)70°C或更冷
注意：不要將冷凍小瓶存放在帶有除霜循環(huán)的冷凍箱中，因?yàn)檫@會使小瓶暴露在升高的溫度下。 氣相液氮（約 - 196°C）是長期儲存（超過一個月）的首選。
| 測試 |
規(guī)范 |
結(jié)果 |
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可行性 |
將樣品材料接種在非選擇性培養(yǎng)基上，孵育并檢查滴度。 結(jié)果≥103cfu /小瓶。 |
6.6 x 106 cfu /小瓶 |
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細(xì)胞和集落形態(tài)整合 |
將樣品材料接種并在非選擇性培養(yǎng)基上生長。 為了形態(tài)符合物種描述（即參考文獻(xiàn)1），目測檢查平板/斜面上的菌落，并在200-400倍放大倍數(shù)下在顯微鏡下檢查細(xì)胞。 文化與適當(dāng)?shù)奈锓N描述一致。 |
通過 |
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純度 |
將樣品材料接種在細(xì)菌生長培養(yǎng)基（液體）中，并在25℃和37℃下孵育至少72小時。 沉淀物（如果有的話）在顯微鏡下以1000倍放大率檢查。 沒有明顯的細(xì)菌細(xì)胞或其他異常細(xì)胞。 |
通過 |
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表型測試 |
樣品由bioMérieuxVITEK?評估.2。結(jié)果與被測生物一致。 |
通過 |
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基因型檢測 |
通過D1 / D2基因區(qū)域測序評估樣品。 結(jié)果與被測試的生物體是可接受的匹配。 |
通過 |
Saccharomyces cerevisiae Meyen ex E.C. Hansen 拉丁名
(ATCC? 9763?) 統(tǒng)一編號
Deposited As Saccharomyces cerevisiae Hansen Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations 菌株別名 NRRL Y-567 [CBS 2978, CBS 5900, CCY 21-4-48, CCY 21-4-54, NCTC 10716, NCTC 7239, NCYC 87, Pattee 6, PCI M-50]
Alternate State Candida robusta Diddens et Lodder
Application 用途 Assay of amphotericin； Assay of amphotericin B fungizone ；Assay of anisomycin； Assay of antifongine ；Assay of candicidin ；Assay of natamycin pimaricin, tennecetin；Assay of nystatin fungicidin； Media testing ；Produces arginase； Produces ethyl alcohol ethanol Food testing ；Pharmaceutical and Personal Care； Produces histone deacetylase ；Produces nicotinic acid niacin ；Sterility testing； Susceptibility testing ；Testing disinfectants Testing fungicides； Produces ethanol from saccharified whole corn mash；Reference strain for performance testing culture media listed by the ISO TC 34 SC 9 Joint Working Group 5 in the ISO 11133 and by the Working Party on Culture Media of the International Committee on Food Microbiology and Hygiene (ICFMH-WPCM)
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏條件
Frozen 冷凍物: -80℃ or colder
Freeze-Dried 凍干物: 2℃ to 8℃
Live Culture 活物: See Propagation Section
Type Strain 模式菌株 no
Preceptrol? yes Ploidy polyploid Ref
Comments 注釋 Invertase activity Oligosaccharide transfer in microsomes Deoxy-trehalose synthesis Morphology On YM agar after 3 days at 25℃, colonies are cream colored, smooth, usually flat, occasionally raised in the centerpart. Older colonies may be slightly tan and opaque. Cells are ovoid, globose, budding, usually isolated or clustered, 3.0-8.0 by 5.0-10.0 ?m.
Medium 培養(yǎng)基 ATCC? Medium 200: YM agar or YM broth
ATCC? Medium 28: Emmons' modification of Sabouraud's agar
ATCC? Medium 1245: YEPD
Growth Conditions 保藏條件
Temperature 培養(yǎng)溫度: 30℃
Atmosphere 需氧情況: Typical aerobicSequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence TATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAACTGGAAATTTTAAAATATTAAAAACT
TTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATG
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG
GGGGCATGCCTGTTTGAGCGTCATTTCCTTCTCAAACATTCTGTTTGGTAGTGAGTGATACTCT
TTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTTTCCAAAGAGAGGTTTCT
CTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTT
TTATACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCTAGGCGAACAA D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGG
CAAAA
GCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGG
GCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGC
GAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAA
TGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGT
GATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGG
GAAGGGCATTTGATCAGACATGGTGTTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGAATCTCG
CATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTC
GGTAA
GTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAAGTCAAGGATG
CTGGCATAATGGTTATATGCCGC
Morphology On YM agar after 3 days at 25℃, colonies are cream colored, smooth, usually flat, occasionally raised in the centerpart. Older colonies may be slightly tan and opaque. Cells are ovoid, globose, budding, usually isolated or clustered, 3.0-8.0 by 5.0-10.0 ?m.