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平臺編號：bio-50953
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國際編號：ATCC VR-1683
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拉丁屬名： Influenza A virus (H1N1)
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
中文名稱暫無
ATCC? Number:VR-1683?
Classification: Orthomyxoviridae, Influenzavirus A
Agent: Influenza A virus (H1N1)
Strain: A/Swine/Iowa/15/30 (TC-adapted)
Original Source: Swine in Iowa, 1930
Depositors: A. P. Kendal and ATCC
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: MDCK (ATCCCCL-34) Alternate Hosts: Chicken embryo, MDCK and Monkey kidney cells (MkK)
Incubation : For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.5℃ in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCCVR-1683 should be grown in serum free media that contains 1 ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Effect : CPE (cell rounding, degeneration, sloughing)
Store at : -70℃ or colder
Comments : Derived by adaptation of egg-passage A/Swine/Iowa/15/30 (ATCCVR-333) to MDCK (ATCCCCL-34) cells. (Note: ATCCVR-333 and ATCCVR-1683 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).
