Mycoplasma hominis (Freundt) Edward
33129™
Product category
Bacteria
Product type
Drug-resistant bacterium
Strain designation
H29
Type strain
No
Isolation source
Clinical isolate
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Preceptrol
No
Characteristics
Susceptibility profile
Resistant
Tetracycline
Comments
Genetic transformation
Handling information
Medium
ATCC Medium 243: Mycoplasma medium
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
Follow instructions as suggested for the culturing of Mollicutes:
PROCEDURES FOR PROPAGATING MOLLICUTES:
Open the vial according to the enclosed instructions.
Using a Pasteur or 1.0 mL pipette, withdraw approximately 0.5 to 1.0 mL from a tube containing 2.5 mL. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the tube. Mix well.
Make serial dilutions by transferring 0.25 mL from the original tube to a tube containing 2.25 mL. Repeat process by transferring 0.25 mL from the second to a third tube, etc. Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze?drying process often inhibits growth.
Use an uninoculated tube of broth to serve as a control.
Plates may be inoculated to check colonial morphology. You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony-forming units. However, not all strains do well on solid medium.
Incubate all tubes and plates under the recommended conditions and appropriate temperature. The time necessary for growth will vary from strain to strain. Growth on plates generally requires additional incubation.
Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.
Tubes and plates are incubated in the atmosphere of 5% CO2. The incubation temperature is 37°C.
Medium to light turbidity will appear in the first few dilution tubes within 48 hours. Additional incubation will be required for colonies to appear on solid medium.
Subsequent, fresh transfers will grow more rapidly than the original rehydrated culture; but usually still requires 48 hours between transfers.
Handling notes
Store vials at freezer temperatures until ready to use.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
History
Deposited as
Mycoplasma hominis (Freundt) Edward
Depositors
G Furness
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
