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平臺(tái)編號(hào)：bio-137594
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國(guó)際編號(hào)：ATCC 59134
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拉丁屬名： phi6-3
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規(guī)格：Freeze-dried
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu) 說(shuō)明書(shū)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
phi6-3
phi6-3
59134 ™
OrganismHomo sapiens, human
Clone typeCloneApplicationsMolecular biology
Product formatFreeze-driedShipping informationbacteria-free lysate
Specific applicationsStandard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C.
The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe.
Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information.
CommentsRestriction digests of the clone give the following sizes (kb): BamHI--25.0, 12.5, 2.0; SalI--19.5, 14.5, 10.0.
phi6-3 contains repeated sequences and must be prehybridized with total human DNA before it will detect single copy bands. Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C.
MspI detects 2 diallelic RFLPs. The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe. Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information.
Mycoplasma contaminationNot detected
Construct size (kb)44.0
Insert size (kb)14.5Type of DNAgenomicGene productDNA Segment, single copy [D11S85]AllelesA1, B2, A2, B1
DepositorsM LittCross referencesGenBank 165643
