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Sideroxydans lithotrophicus
700298 ™

Product category
Bacteria
Strain designation
ES-1
Type strain
Yes
Isolation source
Groundwater tile drain; isolated by D. Emerson
Product format
Frozen
General
Preceptrol
No
Handling information
Medium
ATCC Medium 7266: Modified Wolfe's Mineral Medium Gradient Plate Medium
Temperature
25°C
Atmosphere
Microaerophilic: 3-5% O2, 10% CO2
Handling procedure
1. One frozen vial should be used to start no more than three gradient plates.
2. Autoclave the top and bottom layers for 20 minutes at 121°C.  Cool the top layer in an ice bath while allowing the bottom layer to cool slightly.

3. Pipette 8.5 ml of the bottom layer into a standard Petri dish.  Allow to set a minimum of 15 minutes, but no longer than 30 minutes. 

4. While the bottom layer is setting, adjust the pH of the top layer to between 6.0 and 6.4 by sparging with filter-sterilized CO2.

5. Inoculate the top layer with the vial of S. lithotrophicus and pipette 16 ml over the solidified bottom layer.

6. Place the plates in either GasPak jars with BD BBL CampyPak Plus Microaerophilic system envelopes with palladium catalysts or Mitsubishi AnaeroPack system jars with Pack-MicroAero gas generating envelopes adjusted for the volume of the container.

Handling notes
 

        Growth should be checked by epifluorescent microscopy five days after inoculation.  Syto 13 (Molecular Probes, Eugene, OR) provides good penetration of the Fe-oxides and bright fluorescence.  A 0.25 mM stock solution is prepared in sterile d-H2O that is then diluted 1:5 with the bacterial culture.  Cells are long, helical and vibroid shaped, less than 0.5 um in diameter, and motile during exponential growth.

        Additional volume may be obtained by concentrating cells by centrifugation and using the entire pellet as inoculum for further growth.  For example, three plates may be used as inoculum for 12 plates, and the pellet from 12 plates may be used to inoculate 36 plates.

          Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History
Deposited as
Unidentified bacterium
Depositors
D Emerson
Type of isolate
Environmental
Cross references
GenBank DQ386264 Siderooxidans lithoautotrophicus strain ES-1 16S ribosomal RNA gene, complete sequence
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.