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Anabaena flos-aquae (Lyngbye) Brebisson
22664™

Product category
Bacteria
Type strain
No
Geographical isolation
United States; Wisconsin
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Specific applications
Toxicity testing
Fixes nitrogen
This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E1218-90 where only the taxon is specified.
Preceptrol
No
Characteristics
Comments
Non-axenic
Handling information
Medium
ATCC Medium 625: Gorham's medium for algae
Temperature
25°C
Incubation
Under light intensity of 150 foot candles
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Rehydrate the entire pellet with approximately 0.5 mL of #625 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #625 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
Use several drops of the primary broth tube to inoculate a #625 agar slant.
Incubate at 25°C under light intensity of 150 foot candles. It is helpful to incubate test tubes in a slanted position to increase gas exchange in broth and to enhance exposure to light.
Handling notes
This item is non-axenic

Good growth, indicated by increased pigmentation in the broth or on the slant, should occur after one to two weeks of incubation.  Examine cells microscopically to assure that they are intact and healthy.  At this time additional test tubes or flasks can be inoculated.  A 5% inoculum is recommended (i.e. 5 mL of culture to 100 mL fresh medium).

To minimize change in a culture, it is recommended that a frozen seed stock be established from early passage cells.  This may be accomplished by propagating the strain under ideal conditions, utilizing recommended medium, temperature and light.  Prepare a concentrated cell suspension, after good growth is achieved.  If grown in broth, pellet the cells by centrifugation.  Decant the supernatant and resuspend the pellet in fresh #616 broth using 1/10 or less of the original volume.  For slant cultures, wash cells off the agar surface with a minimal amount of #616 broth so that a concentrated cell suspension is attained.  Add 50% DMSO solution to the concentrated cell suspension so that the final concentration of DMSO in the suspension is 5%.  Dispense small aliquots (0.5 to 1 mL) of the suspension into small sterile vials.  Store the vials at -50°C or below.

When needed, remove vials from storage, thaw contents in a 37°C water bath and inoculate into recommended medium.  A minimum of 0.2 mL of the thawed stock should be used to inoculate 5 mL of broth or 1 agar slant.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.
History
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