Dictyostelium discoideum Raper 拉丁名
ATCC 201386 編號
Strain designation 別名 AX4
Type strain 模式菌株 No
Genome sequenced strainYes
Isolation 分離源 source
Derived from strain AX3, ATCC 28368
Product format 提供形式 Frozen
Storage conditions 保藏溫度 -80°C or colder
CommentsIt was recently found this strain can no longer be maintained and provided as a axenic culture. So it contains its food source bacteria.
Genome sequencing strain (Baylor College of Medicine, USA).
Medium 培養(yǎng)基 ATCC Medium 2219: Corn meal agar, half-strength
Temperature 培養(yǎng)溫度 25°C
IncubationThis culture contains food source bacteria, and thus it is not axenic.Handling procedureFrozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.
If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.
Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).
Storage of frozen material at this temperature will result in the death of the culture.To thaw a frozen ampoule, place in a 25°C-30°C water bath, until just thawed (approximately 5 minutes).
Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 microliter (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Deposited as Dictyostelium discoideum Raper
DepositorsWF Loomis
Chain of custodyATCC <-- WF Loomis <-- W. Nellen
Special collectionEumycetozoan Project
Cross references 參考文獻 GenBank AAFI00000000 Dictyostelium discoideum AX4, whole genome shotgun sequencing project
