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Influenza A virus (H3N2)
VR-1938™

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LEARN ABOUT OUR ENHANCED AUTHENTICATION INITIATIVE 
Influenza A virus (H3N2) strain A/California/2/2014 is propagated in MDCK (NBL-2) cells (ATCC CCL-34). This strain was isolated in 2014 from a human in California and was deposited by the California Department of Public Health. It has applications in infectious and respiratory disease research and in vaccine development.
Product category
Viruses
Product type
Animal virus
Classification
Orthomyxoviridae, Alphainfluenzavirus
Strain designation
A/California/2/2014
Genome sequenced strain
Yes
Isolation source
Isolated from a human on January 16, 2014.
Geographical isolation
United States; California
Applications
Infectious disease research
Respiratory disease research
Vaccine development
Product format
Frozen
Storage conditions
-70°C or colder
General
Specific applications
Respiratory research
Vaccine development
Handling information
Host
MDCK (NBL-2) (ATCC CCL-34)
Effects
CPE; cell rounding; cell sloughing
Complete medium
DMEM (ATCC 30-2002) + 0.18% BSA (Invitrogen™ 15260) + 24 mM HEPES (Invitrogen™ 15630) + 2 µg/mL TPCK-treated trypsin (Sigma-Aldrick® T1426)
Temperature
35°C
Atmosphere
95% Air, 5% CO2
Incubation
2-3 days at 35°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 75% of the monolayer.
Recommendation for infection
Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.1 to 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Key abbreviations
°C, Degrees Celsius
BSA, Bovine serum albumin
CO2, Carbon dioxide
CPE, Cytopathic effect
DMEM, Dulbecco's Modified Eagle's Medium
FBS, Fetal bovine serum
HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
MDCK, Madin Darby canine kidney cells
MOI, Multiplicity of infection
Quality control specifications
Volume
0.5 mL
Verification method
Whole-genome Sequencing
History
Depositors
D Wadford, California Department of Public Health
Type of isolate
Human
Year of origin
2014
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.