Yarrowia lipolytica (Wickerham et al.) van der Walt et von Arx 拉丁名
ATCC 20362 編號
Product category Fungi
Product type Yeast
Strain designation 別名 2002
Type strain 模式菌株 No
Genome sequenced strain Yes
Applications Bioremediation Biofuel production
Product format 提供形式 Freeze-dried
Storage conditions 保藏溫度 2°C to 8°C
Specific applications Degrades petroleum crude oil Potential biocontrol agent: vegetable and mineral oil waste Potential biofuel production agent: biodiesel Preceptrol NoMorphology After 3-5 days growth is butyrous to mycelial and tannish-white in color.
Cells are spheroidal, ellipsoidal to elongate, (3.0-5.0)x(3.3-15.0) μm and single, in pairs or small clusters. Technical information ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC.
Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
Medium 培養(yǎng)基 ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 200: YM agar or YM broth
Temperature 培養(yǎng)溫度 24-26°C
Atmosphere 需氧情況 Aerobic
Handling procedure For freeze-dry (lyophilized) ampoules: Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet.
Stir to form a suspension. Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium.
Include a control that receives no inoculum. Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation.
However, the time necessary for significant growth will vary from strain to strain.
Verification method Whole-genome Sequencing