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Streptococcus pneumoniae (Klein) Chester

700673 ™

 

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Resistant to erythromycin, penicillin, tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole. Representative strain of the Pneumococcal Molecular Epidemiology Network (PMEN).

Product category

Bacteria

Product type

Drug-resistant bacterium

Strain designation

Hungary 19A-6 [HUN663]

Type strain

No

Genome sequenced strain

Yes

Isolation source

Ear

Geographical isolation

Hungary

Applications

Antimicrobial resistance research

Drug development

Infectious disease research

Respiratory disease research

Product format

Freeze-dried

Storage conditions

2°C to 8°C

General

Specific applications

Respiratory research

Preceptrol

No

Characteristics

Antigenic properties

PspA type 12

Serotype

19A

Susceptibility profile

Resistant

Chloramphenicol

Erythromycin

Penicillin

Tetracycline

Trimethoprim-sulfamethoxazole

Comments

Representative strain of the Pneumococcal Molecular Epidemiology Network (PMEN)

Handling information

Medium

ATCC Medium 44: Brain Heart Infusion Agar/Broth

ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood

Temperature

37°C

Atmosphere

95% Air, 5% CO2

Handling procedure

Open vial according to enclosed instructions. 

Using a single tube of #44 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.

Aseptically transfer this aliquot back into the broth tube.  Mix well.

Use several drops of the suspension to inoculate a #260 agar slant and/or plate.

Incubate the tubes and plate at 37°C for 24 hours in atmosphere of 5% CO2. 

Handling notes

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method

Whole-genome Sequencing

History

Deposited as

Streptococcus pneumoniae (Klein) Chester

Depositors

LK McDougal

Chain of custody

ATCC <-- LK McDougal <-- A. Tomasz

Type of isolate

Human

Year of origin

1989

Special collection

NCRR Contract

Cross references

GenBank AX111330 Sequence 2063 from Patent WO0123604.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

 

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

 

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

 

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.