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Aspergillus oryzae var. viridis Murakami, anamorph 拉丁名
ATCC 22788 編號
Deposited As Aspergillus oryzae var. viridis Murakami
Strain Designations 菌種別名 RIB 128 [CBS 819.72, IFO 30113, JCM 2248, NJK 72]
Application 用途 Degrades carbamide
Produces N-desferriferrichrome deferriferrichrome
Produces acid carboxypeptidase
Produces acid protease protease (acid)
Produces amylase, alpha 1A; salivary Amylase, salivary, alpha-1A
Produces galactomannan-protein
Produces glucoamylase
Produces kojic acid
Produces protyrosinase
Produces tyrosinase
Produces urease urea amidolyase
production of urease and degradation of carbamide in sake brewing
Biosafety Level 安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions 培養(yǎng)條件
Frozen 冷凍物 : -80°C or colder
Freeze-Dried 凍干物 : 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes (Samson RA, Gams W. Typification of the species of Aspergillus and associated teleomorphs In Samson RA, Gams W. Advances in Penicillium and Aspergillus systematics. New York, Plenum Press pp. 31-54, 1985.), Ref, Ref
Preceptrol no
Comments 
Rice koji made from this strain turns brown
Taxonomy
Aflatoxin not produced
Medium 培養(yǎng)基 ATCC Medium 336: Potato dextrose agar (PDA)
ATCC Medium 325: Malt extract agar (Blakeslee's formula)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 24°C to 36°C
Atmosphere 需氧情況 : Typical aerobic
Sequenced Data 
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
AAGGATCATTACCGAGTGTAGGGTTCCTAGCGAGCCCAACCTCCCACCCGTGTTTACTGTACCTTAGTTGCTTCGGCGGGCCCGCCATTCATGGCCGCCGGGGGCTCTCAGCCCCGGGCCCGCGCCCGCCGGAGACACCACGAACTCTGTCTGATCTAGTGAAGTCTGAGTTGATTGTATCGCAATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAGTGTGAATTGCAGAATTCCGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCATCAAGCACGGCTTGTGTGTTGGGTCGTCGTCCCCTCTCCGGGGGGGACGGGCCCCAAAGGCAGCGGCGGCACCGCGTCCGATCCTCGAGCGTATGGGGCTTTGTCACCCGCTCTGTAGGCCCGGCCGGCGCTTGCCGAACGCAAATCAATCTTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAG
D1D2 region of the 28S ribosomal RNA gene
CATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGCAGAGGATGCTTCGGGTGCGGCCCCTGTCTAAGTGCCCTGGAACGGGCCGTCAGAGAGGGTGAGAATCCCGTCTGGGATGGGGTGTCCGCGCCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTCGCCTCCAGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCTGGGGGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCTCCCGGAATGTAGTGCCCTCCGGGGCACCTTATAGCCGGGAGTGCAATGCGGCCAGCCTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAATGGTCGTAAACGA
Name of Depositor 寄存者 H Murakami
Isolation 分離基物
Rice koji for making sake, Japan
Cross References 
Nucleotide (GenBank) : D37929 protyrosinase gene, melO
References 參考文獻
Ichishima E, et al. Multiple forms of protyrosinase from Aspergillus oryzae and their mode of activation at pH 3.0. Biochim. Biophys. Acta 786: 25-31, 1984. PubMed: 6424711
Samson RA, Gams W. Typification of the species of Aspergillus and associated teleomorphs In Samson RA, Gams W. Advances in Penicillium and Aspergillus systematics. New York, Plenum Press pp. 31-54, 1985.
. . Rep. Res. Inst. Brew. 143: 5, 1971.
J. Gen. Appl. Microbiol. 17: 281-309, 1971.
. . Biotechnol. Tech. 9: 153-156, 1995.
. . Hakkokogaku 59: 491-500, 1981.
Fujita Y, et al. Molecular cloning and nucleotide sequence of the protyrosinase gene, melO, from Aspergillus oryzae and expression of the gene in yeast cells. Biochim. Biophys. Acta 1261: 151-154, 1995. PubMed: 7893753
. . J. Brew. Soc. Jpn. 78: 475-479, 1983.
Oyashiki H, et al. Screening for strains of Aspergillus oryzae that degrade carbamide for use in sake brewing. J. Ferment. Bioeng. 71: 126-127, 1991.
Nakajima T, Ichishima E. Chemical structure of the galactomannan moiety in the cell wall glycoproteins of Aspergillus oryzae. J. Ferment. Bioeng. 78: 472-475, 1994.
Kumeda Y, Asao T. Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus section Flavi. Appl. Environ. Microbiol. 62: 2947-2952, 1996. PubMed: 8702288
type strain
Samson RA, Gams W. Typification of the species of Aspergillus and associated teleomorphs In Samson RA, Gams W. Advances in Penicillium and Aspergillus systematics. New York, Plenum Press pp. 31-54, 1985.
. . Rep. Res. Inst. Brew. 143: 5, 1971.
J. Gen. Appl. Microbiol. 17: 281-309, 1971.