Kluyveromyces marxianus (EC Hansen) van der Walt 拉丁名
(ATCC? 8554?) 統(tǒng)一編號(hào)
Deposited As Kluyveromyces fragilis (Jorgensen) van der Walt
Strain Designations 別名 [15, type E, ATCC 34439, CBS 5795, CCRC 21480, DSM 5418, NCYC 1426, NRRL Y-1109, UCD 55-61, VTTC-81112]
Alternate State Candida kefyr (Beijerinck) van Uden et Buckley, anamorph
Application 用途 Assay of trichothecenes
Bacterial resistance testing adhesives;
Degrades glucose D-glucose;
Degrades whey permeate;
Ferments pentoses;
Fungus resistance testing adhesives;
Produces acetoin dehydrogenase diacetyl reductase;
Produces beta-galactosidase beta-D-galactosidase;
Produces biomass;
Produces butanediol dehydrogenase acetoin reductase;
Produces ethyl alcohol ethanol;
Produces glycerol glycerin, glycerine;
Produces lactase;
Produces single-cell protein SCP;
Produces biomass on whey permeate of cheddar cheese;
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏條件
Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌株 no
Preceptrol? no
Morphology After 3 days cells are globose, ellipsoidal, to cylindrical in single, pairs, or short chains. Growth is butyrous, glossy, cream-colored to brown.
Medium 培養(yǎng)基 ATCC? Medium 28: Emmons' modification of Sabouraud's agar
ATCC? Medium 200: YM agar or YM broth
ATCC? Medium 1245: YEPD
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度: 24°C to 26°C
Atmosphere 需氧情況: Typical aerobicSequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGATTATGAATGAATAGATTGCTGGGGGAATCGTCTGAACAAGGCCTGCGCTTAATT
GCGCGGCCAGTTCTTGATTCTCTGCTATCAGTTTTCTATTTCTCATCCTAAACACAATGGAGTTCTTTCTCTATGAACTACTTCCCTGGAGAG
CTCGTCTCTCCAGTGGACATAAACACAAACAATATTTTGTATTATGAAAAACTATTATACTATAAAATTTAATATTCAAAACTTTCAACAACG
GATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAATTGCGATATGTATTGTGAATTGCAGATTTTCGTGAATCATCAAATCTTTGAACGC
ACATTGCGCCCTCTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCTTTGGGTTTGGTAGTGAGTGATACTCGTCTC
GGGTTAACTTGAAAGTGGCTAGCCGTTGCCATCTGCGTGAGCAGGGCTGCGTGTCAAGTCTATGGACTCGACTCTTGCACATCTACGTCTTAG
GTTTGCGCCAATTCGTGGTAAGCTTGGGTCATAGAGACTCATAGGTGTTATAAAGACTCGCTGGTGTTTGTCTCCTTGAGGCATACGGCTTTA
ACCAAAACTCTCAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAA
D1D2 region of the 26S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCGTCTTCG
ACGTCCGAGTTGTAATTTGAAGAAGGCGACTTTGTAGCTGGTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGT
GGCGAGGATCCCAGTTATTTGTAAAGTGCTTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAA
TATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAG
GGAAGGGCATTTGATCAGACATGGCGTTTGCTTCGGCTTTCGCTGGGCCAGCATCAGTTTTAGCGGTTGGATAAATCCTCGGGAATGTGGCTCT
GCTTCGGTAGAGTGTTATAGCCCGTGGGAATACAGCCAGCTGGGACTGAGGATTGCGACTTTTGTCAAGGATGCTGGCGTAATGGTTAAATGCCGC
Morphology After 3 days cells are globose, ellipsoidal, to cylindrical in single, pairs, or short chains. Growth is butyrous, glossy, cream-colored to brown.
Name of Depositor 寄存者 Lederle Labs.
Chain of Custody 來(lái)源國(guó)家 ATCC <-- Lederle Labs. <-- N.C. Laffer 15, type E
Isolation 分離基物 Meadowgold creamery, USA
References 參考文獻(xiàn) Crisan EV, Sorensen SG. Production and utilization of lactase. US Patent 4,007,283 dated Feb 8 1977
Schwarz JG, Hang YD. Study of acetoin reductase from Kluyveromyces marxianus. Lett. Appl. Microbiol. 16: 14-16, 1993.
Maleszka R, Schneider H. Fermentation of D-xylose, xylitol, and D-xylulose by yeasts. Can. J. Microbiol. 28: 360-363, 1982. PubMed: 6211222
Madhyastha MS, et al. Optimization of yeast bioassay for trichothecene mycotoxins. J. Food Prot. 57: 490-495, 1994.
chen KC, et al. Search method for the optimal medium for the production of lactase by Kluyveromyces fragilis. Enzyme Microb. Technol. 14: 659-664, 1992.
ASTM International Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4783-01e1.
Mahoney RR, et al. Selection of strain, growth conditions, and extraction procedures for optimum production of lactase from Kluyveromyces fragilis. J. Dairy Sci. 58: 1620-1629, 1975. PubMed: 1194465
Mahoney RR. Number and nature of the sulphydryl groups of beta-galactosidase from Kluyveromyces fragilis. J. Food Biochem. 4: 189-199, 1980.
DeLaney RA, et al. Composition of Saccharomyces fragilis biomass grown on lactose permeate. J. Sci. Food Agric. 26: 1177-1186, 1975.
Inchaurrondo VA, et al. Yeast growth and ?-galactosidase production during aerobic batch cultures in lactose-limited synthetic medium. Process Biochem. 29: 47-54, 1994.
Schwarz JG, Hang YD. Kluyveromyces marxianus: a potential source of diacetyl reductase. World J. Microbiol. Biotechnol. 10: 385-387, 1994.
Rapin JD, et al. Glycerol production by yeast fermentation of whey permeate. Enzyme Microb. Technol. 16: 143-150, 1994.
Freer SN, Detroy RW. Characterization of cellobiose fermentations to ethanol by yeasts. Biotechnol. Bioeng. 25: 541-557, 1983.