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平臺(tái)編號(hào)：bio-116458
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規(guī)格：培養(yǎng)基配方
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用途：/
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
肉肝胃酶消化湯培養(yǎng)基
肉肝胃酶消化湯培養(yǎng)基
【成份】
0054 肉肝胃酶消化湯培養(yǎng)基
Beef 200.0 g
Liver(ox or sheep) 50.0 g
Peptone 10.0 g
Dextrin 10.0 g
HCl 10.0 ml
Pepsin(1:3000) 3.0 g
Distilled water 1.0 L
PREPARATION:
(1)Put the minced beef and liver into the water
add HCl and pepsin in it
then stir.
(2)Digest the meat at 53-55 C for 22-24 hours; stir them every hour at the first 10 hours.
(3)Heat the supernatant to 80 C;add peptone; adjust the pH to 7.6-7.8; boil for 10 min.
(4)Add dextrin after filtration; distri-bute the broth in tubes; covered by paraffin oil
autoclave at 6lb for 20 min.
[note]Add the rusty iron filings about 1/10 volume of the broth
when used for cultivation of Clostridium novyi
【成份】
0054 肉肝胃酶消化湯培養(yǎng)基
Beef 200.0 g
Liver(ox or sheep) 50.0 g
Peptone 10.0 g
Dextrin 10.0 g
HCl 10.0 ml
Pepsin(1:3000) 3.0 g
Distilled water 1.0 L
PREPARATION:
(1)Put the minced beef and liver into the water
add HCl and pepsin in it
then stir.
(2)Digest the meat at 53-55 C for 22-24 hours; stir them every hour at the first 10 hours.
(3)Heat the supernatant to 80 C;add peptone; adjust the pH to 7.6-7.8; boil for 10 min.
(4)Add dextrin after filtration; distri-bute the broth in tubes; covered by paraffin oil
autoclave at 6lb for 20 min.
[note]Add the rusty iron filings about 1/10 volume of the broth
when used for cultivation of Clostridium novyi