【編號】 0148
【名稱】 海水培養(yǎng)基
【成份】 (1)The artificial sea water (ASW)
NaCl 0.4M
MgSO4·7H2O 0.1M
KCl 0.02M
CaCl2·2H2O 0.02M
(2)The basal medium (BM)
tris (hydroxy methyl) aminomethane(tris)-hydrochloride (pH7.5)50mM
NH4Cl 190 mM
K2HPO4·3H2O 0.33mM
FeSO4·7H2O 0.1mM
Half strength ASW
(3)Basal medium agar (BMA)
Was prepared by separately sterilizing and then mixing equal volumes of double strength BM add 15g agar per liter.
(4)Yeast extract broth (YEB)
Was made by supplementing BM with 5g of Difco yeast extract per liter
(5)Yeast extract agar (YEA)
To be prepared by solidifying YEB with 15g of Difco Agar per liter