RH-35（大鼠肝癌細(xì)胞）
1.Origin and General Characteristics
Cell Name RH-35
Organism rattus norvegicus, rat
Age
Tissue
Morphology epithelial
Growth Properties adherent
Descriptions
Biosafety Level
2.Culture Conditions and Handling
Complete Growth Medium DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS
solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells
under an inverted microscope until cell layer is dispersed (usually within 3
to 5 minutes). Cells that are difficult to detach may be placed at 37°C to
facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently
pipetting. Add appropriate aliquots of the cell suspension to new culture
vessels.
Subcultivation Ratio 1:2-1:4
Medium Renewal every 2 to 3 days
Cryopreservation
Freeze medium: 50% basal medium+40% FBS+10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
3.Special Features of the Cell Line
Tumorigenic
Effects
Receptor Expression
Antigen Expression
Gene Expression
Applications