編號：ATCC 9179
批號：63423352
生物：青霉（Penicillium rubens）NRRL 832
產(chǎn)品形式：孢子和菌絲體懸浮在適當?shù)睦鋬霰Ｗo劑中有效期：不適用
儲存條件：2°C至8°C冷凍干燥培養(yǎng)，低濕度; - 冷凍培養(yǎng)70°C或更冷
注意：不要將冷凍小瓶存放在帶有除霜循環(huán)的冷凍箱中，因為這會使小瓶暴露在升高的溫度下。 氣相液氮（約 - 196°C）是長期儲存（超過一個月）的首選。
測試/方法規(guī)格結(jié)果
可行性：將樣品材料接種在非選擇性培養(yǎng)基上，培養(yǎng)并檢查活細胞。文化顯示增長并且是可行的。通過
細胞和集落形態(tài)整合：將樣品材料接種并在非選擇性培養(yǎng)基上生長。為了形態(tài)符合物種描述（即參考文獻1），目測檢查平板/斜面上的菌落，并在200-400倍放大倍數(shù)下在顯微鏡下檢查細胞。文化與適當?shù)奈锓N描述一致。通過
純度：將樣品材料接種在細菌生長培養(yǎng)基（液體）中，并在25℃和37℃下培養(yǎng)最少48小時。
沉淀物：在顯微鏡下以1000倍放大率檢查。沒有明顯的細菌細胞或其他異常細胞。通過
基因型檢測通過ITS和部分28S測序評估樣品。結(jié)果與被測試的生物體是可接受的匹配。通過
ATCC?培養(yǎng)基28：Emmons對Sabouraud瓊脂ATCC?培養(yǎng)基312的修飾：Czapek的瓊脂
ATCC?培養(yǎng)基336：馬鈴薯葡萄糖瓊脂（PDA）
生長條件溫度：25°C至30°C氣氛：典型的有氧運動
推薦程序
對于freezedry（凍干）安瓿：
1.從單個無菌蒸餾水試管（5至6 mL）中，用無菌移液管取出約0.5至1.0 mL，直接涂于顆粒上。攪拌形成懸浮液。
2.將懸浮液無菌轉(zhuǎn)移回無菌蒸餾水的試管中。
3.讓試管在室溫（25℃）下靜置至少2小時;更長時間（例如，過夜）再水化可以增加一些真菌的活力。
4.將懸浮液混合均勻。使用幾滴（或根據(jù)需要進行稀釋）接種推薦的固體或液體培養(yǎng)基。包括不接受接種物的對照。
5.在推薦的繁殖條件下孵育接種物。
6.定期檢查接種物/菌株的生長情況。在孵育2至3天后，活力通常是顯著的。然而，顯著增長所需的時間因品種而異。
Penicillium rubens Biourge拉丁名 (ATCC? 9179?) 統(tǒng)一編號
Deposited As Penicillium notatum Westling
Strain Designations 菌株別名 NRRL 832 [ATCC 7813, B 69, CBS 197.46, IMI 17968, QM 940]
Application 用途 Produces penicillin； Produces phosphogluconate dehydrogenase 6-phosphogluconate dehydrogenase
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏條件
Frozen 冷凍物: -80℃ or colder
Freeze-Dried 凍干物: 2℃ to 8℃
Live Culture 活物: See Propagation Section
Type Strain 模式菌株 no
Preceptrol? yes
Comments Prototype strain for production of penicillin in submerged culture The strain was reidentified as Penicillium rubens based on the multiloci DNA sequence analysis
Medium 培養(yǎng)基 ATCC? Medium 28: Emmons' modification of Sabouraud's agar
ATCC? Medium 312: Czapek's agar
ATCC? Medium 336: Potato dextrose agar (PDA)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 25℃ to 30℃
Atmosphere 需氧情況: Typcial aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACCGAGTGAGGGCCCTCTGGGTCCAACCTCCCACCCGTGTTTATT
TTACCTTGTTGCTTCGGCGGGCCCGCCTTAACTGGCCGCCGGGGGGCTTACGCCCCCGGGCCCGCGCCCGCCGAAGA
CACCCTCGAACTCTGTCTGAAGATTGTAGTCTGAGTGAAAATATAAATTATTTAAAACTTTCAACAACGGATCTCTTGGTTCCG
GCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAAATTCAGTGAATCATCGAGTCTTTGAACGCACATTG
CGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTTCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCGTC
CTCCGATCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTTGTCACCC
GCTCTGTAGGCCCGGCCGGCGCTTGCCGATCAACCCAAATTTTTATCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCT
GAACTTAAGCATATCAATAA Beta-tubulin (TUB2) gene, partial sequence GATCCCCTCCAGACGCGTCTTTTGTTTTCATATGGCCCCTGAGCCACGACCCCACCCCCAACAGATCTTTTGCTAAC
TTGCTCTA
GGTTCACCTCCAAACCGGCCAGTGTGTAAGTTCAACATGGAACATTCTTGGAAACATTCTTCGATTCGTGGGACT
AAACTGGAATTGGGTTATAGGGTAACCAAATTGGTGCCGCTTTCTGGTAAGTCTCGGAGCTTTTTTTTTCGCGTTGGG
TATCAATTGACAAGTTGCTAACTGGATTACAGGCAAACCATCTCTGGCGAGCACGGTCTCGATGGCGATGGACAGTA
AGTTTAACAGTGATAGGGATTCTGGTGGATCACACGTCTGATATCTTGCTAGGTACAATGGTACCTCCGACCTCCAGC
TCGAGCGTATGAACGTCTACTTCAACCATGTGAGTACAATGACTGGGAATCGATTGATTGTGCATCATCTGATCGGAC
GTTTTTCTTTGACAATCTAGGCCAGCGGTGACAAGTACGTTCCCCGTGCCGTTCTGGTCGATTTGGAGCCCGGTACC
ATGGATGCTGTCCGCTCCGGTCCCTTCGGCAAGCTTTTCCGCCCCGACAACTTCGTCTTCGGTCAGTCCGGTGCTG
GTAACAACTGGGCCAAGGGTCACT D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATT
TGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGTAGAGGATGCTTCGGGAGCGGTCCCCAT
CTAAGTGCCCTGGAACGGGACGTCATAGAGGGTGAGAATCCCGTATGGGATGGGGTGTCCGCGCCCGTGTGAAGC
TCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCGG
AGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGCACGTGAAATTG
TTGAAAGGGAAGCGCTTGCGACCAGACTCGCTCGCGGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCCGCGG
GCGGGCCAGCGTCGGTTTGGGCGGTCGGTCAAAGGCCCTCGGAAGGTAACGCCCCTAGGGGCGTCTTATAGCCG
AGGGTGCAATGCGACCTGCCTAGACCGAGGAACGCGCTTCGGCTCGGACGCTGGCATAATGGTCGTAAACGAC
Name of Depositor 寄存人 NRRL
Chain of Custody 來源國家 ATCC <-- NRRL <-- C Thom B 69
Isolation 分離基物 Contaminant in culture labeled 'P. lacticus Maze & Perrier' from Ph. Biourge 69, Washington, DC
Cross References Nucleotide (GenBank) : KU729086 ITS including 5.8S rRNA gene
Nucleotide (GenBank) : KU729184 D1/D2 region of 28S rRNA gene
Nucleotide (GenBank) : KU896998 beta-tubulin (TUB2) gene
References參考文獻 Houbraken J, et al. New penicillin-producing Penicillium species and an overview of section Chrysogena. Persoonia 29: 78-100, 2012. PubMed: 23606767
Miller HM, Shepherd MG. Purification and properties of 6-phosphogluconate dehydrogenase from Penicillium duponti and Penicillium notatum. Can. J. Microbiol. 18: 1289-1298, 1972. PubMed: 5052896
Moyer AJ, Coghill RD. Penicillin. IX. The laboratory scale production of penicillin in submerged cultures by Penicillium notatum Westling (NRRL 832). J. Bacteriol. 51: 79-93, 1946.
Chou H, et al. Transaldolases are novel and immunoglobulin E cross-reacting fungal allergens. Clin. Exp. Allergy 41: 739-749, 2011. PubMed: 21488999
Houbraken J, Frisvad JC, Samson RA. Fleming's penicillin producing strain is not Penicillium chrysogenum but P. rubens. IMA Fungus 2: 87-95, 2011. PubMed: 22679592
Chow LP, et al. Characterization of Pen n 13, a major allergen from the mold Penicillium notatum. Biochem. Biophys. Res. Commun. 269: 14-20, 2000. PubMed: 10694469