Species:Escherichia coli
Group:K12
Strain designation:MG1655 SEGA032
Accession number:LMBP 12868
Depositor:A.Ehrmann1 and Prof.Dr M.Nørholm1.It was constructed by C.Bayer1,A.Ehrmann1 and M.Rennig1.
1 The Novo Nordisk Foundation Center for Biosustainability,Technical University of Denmark,Horsholm,Denmark
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Other culture collection numbers:SEGA 032
Medium:LB-Lennox
Selection marker:tetracycline(10µg/ml)
Temperature:37°C
Genotype:F-λ-ilvG-rfb-50 rph-1 glmS-pstS::Ptrc-low-PelB-sfgfp-tetA(Source:http://openwetware.org/wiki/E._coli_genotypes#MG1655+Bayer et al.,2021)
SEGA sequence:p12868.gb
Phenotype:tetR
Properties:The strain was created based on Escherichia coli K12 MG1655,by integrating the SEGA landing pad between the glmS and pstS loci.Counter-selection is posssible through sensitivity to NiCl2(50µM).
Further information:This host strain is intended for simplistic bacterial genome engineering,using the SEGA genome architecture.
Original reference:Bayer et al.,Nat.Commun.12(2021),5876[DOI:10.1038/s41467-021-26155-5]
Restricted use:-BCCM MTA
-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Refer in your Materials and Methods:
Escherichia coli K12 MG1655 SEGA032(LMBP 12868)is available at BCCM/GeneCorner.It was deposited by A.Ehrmann and Prof.Dr M.Nørholm and was published in Bayer et al.,2021.
