Species:Escherichia coli
Group:K12
Strain designation:SM10(λpir)
Accession number:LMBP 3889
Depositor:Dr J.J.Mekalanos1
1 Department of Microbiology and Molecular Genetics,Harvard Medical School,Boston,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:kanamycin(50μg/ml)
Temperature:37°C
Genotype:thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmλpir
Phenotype:KanR
Properties:Donor strain(mobilizing strain)carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.Useful for mobilizing mobRP4 plasmids.
Further information:The presence ofλpir allows the replication of a defective R6K suicide plasmid and derivatives thereof.
Original reference:-
Related reference:Simon et al.,Biotechnology 1(1983),784-791
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 SM10(λpir)(LMBP 3889)is available at BCCM/GeneCorner.It was deposited by Dr J.J.Mekalanos.
Group:K12
Strain designation:SM10(λpir)
Accession number:LMBP 3889
Depositor:Dr J.J.Mekalanos1
1 Department of Microbiology and Molecular Genetics,Harvard Medical School,Boston,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:kanamycin(50μg/ml)
Temperature:37°C
Genotype:thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmλpir
Phenotype:KanR
Properties:Donor strain(mobilizing strain)carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.Useful for mobilizing mobRP4 plasmids.
Further information:The presence ofλpir allows the replication of a defective R6K suicide plasmid and derivatives thereof.
Original reference:-
Related reference:Simon et al.,Biotechnology 1(1983),784-791
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 SM10(λpir)(LMBP 3889)is available at BCCM/GeneCorner.It was deposited by Dr J.J.Mekalanos.
