Species:Escherichia coli
Group:K12
Strain designation:WG389
Accession number:LMBP 12441
Depositor:This host strain was deposited by Dr D.E.Culham1 and Prof.Em.Dr J.M.Wood1.
1 Department of Molecular and Cellular Biology,University of Guelph,Guelph,Canada
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox+pro(25 mM)
Selection marker:-
Temperature:37°C
Genotype:F-trp lacZ rpsL thi?putPA101?(proU)600?(proP-melAB)212?(brnQ phoA proC)
Phenotype:StrR
Properties:This is a proline auxotroph lacking all known proline uptake systems.
Further information:Resistance to streptomycin has been experimentally confirmed by BCCM/GeneCorner.
Original reference:Culham et al.,J.Mol.Biol.229(1993),268-276[PMID:8421314][DOI:10.1006/jmbi.1993.1030]
Restricted use:-BCCM MTA
-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Refer in your Materials and Methods:
Escherichia coli K12 WG389(LMBP 12441)is available at BCCM/GeneCorner.It was deposited by This host strain was deposited by Dr D.E.Culham and Prof.Em.Dr J.M.Wood and was published in Culham et al.,1993.
Group:K12
Strain designation:WG389
Accession number:LMBP 12441
Depositor:This host strain was deposited by Dr D.E.Culham1 and Prof.Em.Dr J.M.Wood1.
1 Department of Molecular and Cellular Biology,University of Guelph,Guelph,Canada
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox+pro(25 mM)
Selection marker:-
Temperature:37°C
Genotype:F-trp lacZ rpsL thi?putPA101?(proU)600?(proP-melAB)212?(brnQ phoA proC)
Phenotype:StrR
Properties:This is a proline auxotroph lacking all known proline uptake systems.
Further information:Resistance to streptomycin has been experimentally confirmed by BCCM/GeneCorner.
Original reference:Culham et al.,J.Mol.Biol.229(1993),268-276[PMID:8421314][DOI:10.1006/jmbi.1993.1030]
Restricted use:-BCCM MTA
-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Refer in your Materials and Methods:
Escherichia coli K12 WG389(LMBP 12441)is available at BCCM/GeneCorner.It was deposited by This host strain was deposited by Dr D.E.Culham and Prof.Em.Dr J.M.Wood and was published in Culham et al.,1993.
