Species:Lactococcus lactis
Group:
Strain designation:subsp.cremoris MG1363
Accession number:LMBP 3019
Depositor:Prof.Dr E.Remaut1 2
1 VIB-UGent Center for Inflammation Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
It was obtained from Dr J.Wells3
3 Microbiol Genetics and Physiology Section,Institute of Food Research,Colney,Norwich,UK
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:M17+0.5%glucose(GM17)
Selection marker:/
Temperature:28°C
Cultivation remarks:Cultivate under unaerated conditions for at least 20 hours.
Genotype:-
Phenotype:-
Properties:MG1363 is a derivative of L.lactis subsp.cremoris NCDO 712,which was cured of all resident plasmids.Consequently,it cannot ferment lactose and does not produce extracellular protease.
Further information:The full genome sequence of this strain is available at the EMBL Nucleotide Sequence Database under accession number AM406671.1.
Original reference:Gasson,J.Bacteriol.154(1983),1-9[PMID:6403500]
Restricted use:-BCCM MTA
Cultivation remarks:Cultivate under unaerated conditions for at least 20 hours.
Refer in your Materials and Methods:
Lactococcus lactis subsp.cremoris MG1363(LMBP 3019)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Gasson,1983.
Group:
Strain designation:subsp.cremoris MG1363
Accession number:LMBP 3019
Depositor:Prof.Dr E.Remaut1 2
1 VIB-UGent Center for Inflammation Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
It was obtained from Dr J.Wells3
3 Microbiol Genetics and Physiology Section,Institute of Food Research,Colney,Norwich,UK
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:M17+0.5%glucose(GM17)
Selection marker:/
Temperature:28°C
Cultivation remarks:Cultivate under unaerated conditions for at least 20 hours.
Genotype:-
Phenotype:-
Properties:MG1363 is a derivative of L.lactis subsp.cremoris NCDO 712,which was cured of all resident plasmids.Consequently,it cannot ferment lactose and does not produce extracellular protease.
Further information:The full genome sequence of this strain is available at the EMBL Nucleotide Sequence Database under accession number AM406671.1.
Original reference:Gasson,J.Bacteriol.154(1983),1-9[PMID:6403500]
Restricted use:-BCCM MTA
Cultivation remarks:Cultivate under unaerated conditions for at least 20 hours.
Refer in your Materials and Methods:
Lactococcus lactis subsp.cremoris MG1363(LMBP 3019)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Gasson,1983.
