基本信息
啟動(dòng)子: CMV
復(fù)制子: pUC
質(zhì)粒大小: 7567bp
原核抗性: Amp
篩選標(biāo)記: EGFP
克隆菌株: Stbl3
培養(yǎng)條件: 37度
質(zhì)粒屬性
載體宿主: 哺乳細(xì)胞,慢病毒
載體用途: 干擾沉默
基因種屬:
基因類型: shRNA
原核抗性: Amp
真核抗性:
熒光蛋白: 綠色
質(zhì)粒簡(jiǎn)介
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
pSico shRNA cloning protocol