ATCC 10379=NCTC 5414 =CCUG 37985=CIP 101126= LMG 18984= NCTC 12968
培養(yǎng)基編號(hào):35
培養(yǎng)溫度:37℃,5% CO2
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培養(yǎng)基信息
培養(yǎng)基編號(hào):35
名稱:Blond Agar (血瓊脂培養(yǎng)基)
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備注:Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g NaCl 5g NaH2PO4 2.5gSodium citrate (檸檬酸鈉) 5g Brain infusion (腦浸汁) 800mlPig heart infusion (豬心浸) 200ml Sleep Blood (羊血) 10%pH 7.2 [Note]:Preparation of pig brain and pig heart infusion:(1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml;(2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;(3) Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;(4) Cool and filtrate through gauze . [注]豬心和豬腦浸汁法:(1)新鮮豬心除去心頭和脂肪,攪碎100克加蒸餾水至1000毫升;(2)新鮮豬腦除去血管,取200克加蒸餾水至1000毫升;(3)50℃保溫1小時(shí),然后煮開(kāi)15分鐘;(4)冷卻后用紗布過(guò)濾備用。