| 啟動(dòng)子 | CMV |
| 復(fù)制子 | pUC ori |
| 終止子 | β-globin poly(A) |
| 質(zhì)粒大小 | 8890bp |
| 原核抗性 | Amp |
| 克隆菌株 | Stbl3 |
| 培養(yǎng)條件 | 37℃，有氧 LB |
| 表達(dá)宿主 | 哺乳細(xì)胞 |
| 誘導(dǎo)方式 | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
| 5'測(cè)序引物 | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3'測(cè)序引物 | 根據(jù)序列設(shè)計(jì)引物 |
| 質(zhì)粒簡(jiǎn)介 | This 3rd generation LV packaging plasmid includes gag, coding for the virion main structural proteins; pol, responsible for the retrovirus-specific enzymes; and RRE, a binding site for the Rev protein which facilitates export of the RNA from the nucleus. This plasmid does not include Rev. The third generation packaging system offers maximal biosafety but is more cumbersome, as it involves the transfection of four different plasmids in the producer cells. |
