-
平臺編號：bio-132102
-
拉丁屬名： DH5α，BL21
-
規(guī)格：2ml甘油管 20ul質粒
-
服務費用：
加載中…… - 訂購
-
注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
pCMV-HA-UB
| 啟動子 | CMV promoter | |
| 復制子 | ori | |
| 終止子 | SV40 poly(A) signal | |
| 質粒分類 | 基因文庫質粒；人源質粒；泛素化系列質粒 | |
| 質粒大小 | 3997bp | |
| 質粒標簽 | N-HA | |
| 原核抗性 | 氨芐青霉素Amp | |
| 篩選標記 | 無 | |
| 克隆菌株 | DH5α | |
| 培養(yǎng)條件 | LB，37℃ | |
| 表達宿主 | 哺乳細胞 | |
| 誘導方式 | 無需誘導 | |
| 5'測序引物 | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3'測序引物 | Sv40-polyA-R：GAAATTTGTGATGCTATTGC | |
| 質粒簡介 |
人類Ubiquiitin基因有四個，編碼同一個蛋白，稱為Ubiquitin分子，這四個基因的mRNA水平存在些許差異，但是蛋白序列是一樣的。其中最常見的是Ubiquitin C（UBC）、Ubiquitin B（UBB）基因，UBC mRNA編碼六個重復的Ubiquitin分子，UBB編碼四個重復的Ubquitin分子，所以外源克隆的HA-UB一般只克隆了一個ub分子，也就只有76個氨基酸，如果您需要連續(xù)幾個Ub的那種，可以委托我們做亞克隆，HA-ub(n),n=1.2.3.4.5.6..... Homo sapiens ubiquitin C (UBC), mRNA，NCBI Reference Sequence: NM_021009.6 |
|
| 質粒序列 |
LOCUS Exported 3997 bp ds-DNA circular SYN 11-MAR-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMV-HA-UB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3997) AUTHORS . TITLE Direct Submission JOURNAL Exported Saturday, March 11, 2017 FEATURES Location/Qualifiers source 1..3997 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 27..330 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 331..534 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" intron 672..768 /note="SV40 intron" /note="modified SV40 intron with splice donor and acceptor sites" CDS 832..858 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" misc_feature 886..1113 /note="UB" polyA_signal 1136..1270 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind complement(1381..1397) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 1405..1421 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1429..1459) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 1474..1495 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1783..2371) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2542..3402) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3403..3507) /gene="bla" /note="AmpR promoter" primer_bind 3981..3997 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc 61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta 541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac 601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag 661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg 721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa 781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac 841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcgcatgca gatcttcgtg 901 aagaccctga ctggtaagac catcactctc gaagtggagc cgagtgacac cattgagaat 961 gtcaaggcaa agatccaaga caaggaaggc atccctcctg accagcagag gttgatcttt 1021 gctgggaaac agctggaaga tggacgcacc ctgtctgact acaacatcca gaaagagtcc 1081 accctgcacc tggtcctccg tctcagaggt gggagtgcgg taccgcggcc gcggggatcc 1141 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 1201 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 1261 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 1321 ggaggttttt tcggatcctc tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg 1381 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 1441 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 1501 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 1561 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 1621 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 1681 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 1741 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 1801 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 1861 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 1921 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 1981 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 2041 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 2101 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 2161 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 2221 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 2281 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 2341 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 2401 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 2461 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 2521 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 2581 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 2641 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 2701 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 2761 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 2821 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 2881 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 2941 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 3001 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 3061 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 3121 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 3181 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 3241 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 3301 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 3361 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 3421 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 3481 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 3541 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 3601 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 3661 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 3721 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 3781 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 3841 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3901 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3961 gttttcccag tcacgacgtt gtaaaacgac ggccagt |
