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GEX-6P-1
    GEX-6P-1
  • 平臺編號:bio-126808
  • 質(zhì)粒信息: GEX-6P-1
  • 規(guī)格:2ml甘油管 20ul質(zhì)粒
  • 用途:/
  • 服務(wù)費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

 啟動子  Tac/lac
 復(fù)制子  ColE1 ori
 質(zhì)粒分類  大腸桿菌載體;PGEX系列表達質(zhì)粒
 質(zhì)粒大小  4984bp
 質(zhì)粒標簽  N-GST,N-PP/3C
 原核抗性  氨芐青霉素Amp
 克隆菌株  DH5α
 培養(yǎng)條件  37℃,有氧,LB
 表達宿主  大腸桿菌
 誘導(dǎo)方式  IPTG或乳糖及其類似物
 5'測序引物  pGEX5: GGGCTGGCAAGCCACGTTTGGTG
 3'測序引物  pGEX3: CCGGGAGCTGCATGTGTCAGAGG
 備注  可用GST親和柱純化重組蛋白
 質(zhì)粒簡介  There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 μl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 μl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty gravity flow columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below.

 

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