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平臺編號：bio-126808
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質(zhì)粒信息： GEX-6P-1
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規(guī)格：2ml甘油管 20ul質(zhì)粒
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用途：/
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
GEX-6P-1
| 啟動子 | Tac/lac |
| 復(fù)制子 | ColE1 ori |
| 質(zhì)粒分類 | 大腸桿菌載體；PGEX系列表達(dá)質(zhì)粒 |
| 質(zhì)粒大小 | 4984bp |
| 質(zhì)粒標(biāo)簽 | N-GST，N-PP/3C |
| 原核抗性 | 氨芐青霉素Amp |
| 克隆菌株 | DH5α |
| 培養(yǎng)條件 | 37℃，有氧，LB |
| 表達(dá)宿主 | 大腸桿菌 |
| 誘導(dǎo)方式 | IPTG或乳糖及其類似物 |
| 5'測序引物 | pGEX5: GGGCTGGCAAGCCACGTTTGGTG |
| 3'測序引物 | pGEX3: CCGGGAGCTGCATGTGTCAGAGG |
| 備注 | 可用GST親和柱純化重組蛋白 |
| 質(zhì)粒簡介 | There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 μl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 μl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty gravity flow columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below. |
