啟動子 | Tac/lac |
復(fù)制子 | ColE1 ori |
質(zhì)粒分類 | 大腸桿菌載體;PGEX系列表達質(zhì)粒 |
質(zhì)粒大小 | 4984bp |
質(zhì)粒標簽 | N-GST,N-PP/3C |
原核抗性 | 氨芐青霉素Amp |
克隆菌株 | DH5α |
培養(yǎng)條件 | 37℃,有氧,LB |
表達宿主 | 大腸桿菌 |
誘導(dǎo)方式 | IPTG或乳糖及其類似物 |
5'測序引物 | pGEX5: GGGCTGGCAAGCCACGTTTGGTG |
3'測序引物 | pGEX3: CCGGGAGCTGCATGTGTCAGAGG |
備注 | 可用GST親和柱純化重組蛋白 |
質(zhì)粒簡介 | There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 μl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 μl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty gravity flow columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below. |