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平臺(tái)編號(hào)：bio-114927
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質(zhì)粒信息： pAdEasy-1
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規(guī)格：2ml甘油管 20ul質(zhì)粒
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用途：/
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
pAdEasy-1
基本信息
復(fù)制子: pUC
質(zhì)粒分類: 病毒系列，腺病毒載體
質(zhì)粒大小: 33477bp
原核抗性: Amp
克隆菌株: Stbl3
培養(yǎng)條件: 30度
表達(dá)宿主: 哺乳細(xì)胞
誘導(dǎo)方式: 無須誘導(dǎo)，瞬時(shí)表達(dá)
5'測(cè)序引物: M13F:TGTAAAACGACGGCCAGT
3'測(cè)序引物: 根據(jù)序列設(shè)計(jì)引物
質(zhì)粒屬性
載體宿主： 哺乳細(xì)胞,腺病毒
載體用途： 蛋白表達(dá)
基因種屬：
基因類型： ORF
原核抗性： Amp
真核抗性：
熒光蛋白：
質(zhì)粒簡(jiǎn)介
The plasmid pAdEasy-1, containing most of the human adenovirus serotype 5 (Ad5) genome, is deleted for the genes E1 and E3. The removal of these two viral genes creates space for foreign DNA and eliminates selfreplication capabilities. The E1 deletion renders the viruses defective for replication and incapable of producing infectious viral particles in target cells (provided there is no complementation by the host cell); the E3 region encodes proteins involved in evading host immunity and is dispensable. The deletion of both genes creates room for up to 7.5 kb of foreign DNA that can be inserted into the Ad5 genome. The E1 gene, which is necessary for production of viral particles, is provided in trans by AD-293 cells. pAdEasy-1 carries the ampicillin resistance gene, which is lost after recombination with a shuttle vector.
質(zhì)粒圖譜
質(zhì)粒序列
復(fù)制子: pUC
質(zhì)粒分類: 病毒系列，腺病毒載體
質(zhì)粒大小: 33477bp
原核抗性: Amp
克隆菌株: Stbl3
培養(yǎng)條件: 30度
表達(dá)宿主: 哺乳細(xì)胞
誘導(dǎo)方式: 無須誘導(dǎo)，瞬時(shí)表達(dá)
5'測(cè)序引物: M13F:TGTAAAACGACGGCCAGT
3'測(cè)序引物: 根據(jù)序列設(shè)計(jì)引物
質(zhì)粒屬性
載體宿主： 哺乳細(xì)胞,腺病毒
載體用途： 蛋白表達(dá)
基因種屬：
基因類型： ORF
原核抗性： Amp
真核抗性：
熒光蛋白：
質(zhì)粒簡(jiǎn)介
The plasmid pAdEasy-1, containing most of the human adenovirus serotype 5 (Ad5) genome, is deleted for the genes E1 and E3. The removal of these two viral genes creates space for foreign DNA and eliminates selfreplication capabilities. The E1 deletion renders the viruses defective for replication and incapable of producing infectious viral particles in target cells (provided there is no complementation by the host cell); the E3 region encodes proteins involved in evading host immunity and is dispensable. The deletion of both genes creates room for up to 7.5 kb of foreign DNA that can be inserted into the Ad5 genome. The E1 gene, which is necessary for production of viral particles, is provided in trans by AD-293 cells. pAdEasy-1 carries the ampicillin resistance gene, which is lost after recombination with a shuttle vector.
質(zhì)粒圖譜
質(zhì)粒序列
LOCUS Exported 33477 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 33477)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..33477
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1037..1228
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
misc_feature 1330..1470
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1656..2244)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2415..3275)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3276..3380)
/gene="bla"
/note="AmpR promoter"
misc_feature 6972..7075
/note="Ad SA"
/note="adenovirus major late transcript splice acceptor
(Friedrich and Soriano, 1991)"
CDS complement(14168..14179)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
repeat_region 33121..33223
/note="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
ORIGIN
1 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
61 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
121 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
181 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
241 gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
301 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
361 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
421 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc
481 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg
541 gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt
601 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg
661 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat
721 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat
781 tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt
841 attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac
901 cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg
961 tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc
1021 ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat
1081 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc
1141 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg
1201 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
1261 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
1321 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
1381 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
1441 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
1501 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
1561 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
1621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
1681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
1741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
1801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
1861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
1921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
1981 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
2041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
2101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
2161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
2221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
2281 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
2341 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
2401 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
2461 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
2521 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
2581 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
2641 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
2701 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
2761 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
2821 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
2881 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
2941 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
3001 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
3061 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
3121 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
3181 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
3241 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
3301 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
3361 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgtcta gctacgaatt
3421 cttcgacagc ttcgaaacta gaatcgattt cgaaactagc ttaagggtgg gaaagaatat
3481 ataaggtggg ggtcttatgt agttttgtat ctgttttgca gcagccgccg ccgccatgag
3541 caccaactcg tttgatggaa gcattgtgag ctcatatttg acaacgcgca tgcccccatg
3601 ggccggggtg cgtcagaatg tgatgggctc cagcattgat ggtcgccccg tcctgcccgc
3661 aaactctact accttgacct acgagaccgt gtctggaacg ccgttggaga ctgcagcctc
3721 cgccgccgct tcagccgctg cagccaccgc ccgcgggatt gtgactgact ttgctttcct
3781 gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc cgcgatgaca agttgacggc
3841 tcttttggca caattggatt ctttgacccg ggaacttaat gtcgtttctc agcagctgtt
3901 ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc cctcccaatg cggtttaaaa
3961 cataaataaa aaaccagact ctgtttggat ttggatcaag caagtgtctt gctgtcttta
4021 tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg tctcggtcgt tgagggtcct
4081 gtgtattttt tccaggacgt ggtaaaggtg actctggatg ttcagataca tgggcataag
4141 cccgtctctg gggtggaggt agcaccactg cagagcttca tgctgcgggg tggtgttgta
4201 gatgatccag tcgtagcagg agcgctgggc gtggtgccta aaaatgtctt tcagtagcaa
4261 gctgattgcc aggggcaggc ccttggtgta agtgtttaca aagcggttaa gctgggatgg
4321 gtgcatacgt ggggatatga gatgcatctt ggactgtatt tttaggttgg ctatgttccc
4381 agccatatcc ctccggggat tcatgttgtg cagaaccacc agcacagtgt atccggtgca
4441 cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg aagaacttgg agacgccctt
4501 gtgacctcca agattttcca tgcattcgtc cataatgatg gcaatgggcc cacgggcggc
4561 ggcctgggcg aagatatttc tgggatcact aacgtcatag ttgtgttcca ggatgagatc
4621 gtcataggcc atttttacaa agcgcgggcg gagggtgcca gactgcggta taatggttcc
4681 atccggccca ggggcgtagt taccctcaca gatttgcatt tcccacgctt tgagttcaga
4741 tggggggatc atgtctacct gcggggcgat gaagaaaacg gtttccgggg taggggagat
4801 cagctgggaa gaaagcaggt tcctgagcag ctgcgactta ccgcagccgg tgggcccgta
4861 aatcacacct attaccggct gcaactggta gttaagagag ctgcagctgc cgtcatccct
4921 gagcaggggg gccacttcgt taagcatgtc cctgactcgc atgttttccc tgaccaaatc
4981 cgccagaagg cgctcgccgc ccagcgatag cagttcttgc aaggaagcaa agtttttcaa
5041 cggtttgaga ccgtccgccg taggcatgct tttgagcgtt tgaccaagca gttccaggcg
5101 gtcccacagc tcggtcacct gctctacggc atctcgatcc agcatatctc ctcgtttcgc
5161 gggttggggc ggctttcgct gtacggcagt agtcggtgct cgtccagacg ggccagggtc
5221 atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct gggtcacggt gaaggggtgc
5281 gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg tcctgctggt gctgaagcgc
5341 tgccggtctt cgccctgcgc gtcggccagg tagcatttga ccatggtgtc atagtccagc
5401 ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg aggaggcgcc gcacgagggg
5461 cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa ataccgattc cggggagtag
5521 gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca cgagccaggt gagctctggc
5581 cgttcggggt caaaaaccag gtttccccca tgctttttga tgcgtttctt acctctggtt
5641 tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt ccgtgtcccc gtatacagac
5701 ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct cgtatagaaa ctcggaccac
5761 tctgagacaa aggctcgcgt ccaggccagc acgaaggagg ctaagtggga ggggtagcgg
5821 tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa gacacatgtc gccctcttcg
5881 gcatcaagga aggtgattgg tttgtaggtg taggccacgt gaccgggtgt tcctgaaggg
5941 gggctataaa agggggtggg ggcgcgttcg tcctcactct cttccgcatc gctgtctgcg
6001 agggccagct gttggggtga gtactccctc tgaaaagcgg gcatgacttc tgcgctaaga
6061 ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg
6121 agggtggccg catccatctg gtcagaaaag acaatctttt tgttgtcaag cttggtggca
6181 aacgacccgt agagggcgtt ggacagcaac ttggcgatgg agcgcagggt ttggtttttg
6241 tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca cgtattcgcg cgcaacgcac
6301 cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca ggtgcacgcg ccaaccgcgg
6361 ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc cgcgtaggcg ctcgttggtc
6421 cagcagaggc ggccgccctt gcgcgagcag aatggcggta gggggtctag ctgcgtctcg
6481 tccggggggt ctgcgtccac ggtaaagacc ccgggcagca ggcgcgcgtc gaagtagtct
6541 atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc gggcggcaag cgcgcgctcg
6601 tatgggttga gtgggggacc ccatggcatg gggtgggtga gcgcggaggc gtacatgccg
6661 caaatgtcgt aaacgtagag gggctctctg agtattccaa gatatgtagg gtagcatctt
6721 ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt gcgagggagc gaggaggtcg
6781 ggaccgaggt tgctacgggc gggctgctct gctcggaaga ctatctgcct gaagatggca
6841 tgtgagttgg atgatatggt tggacgctgg aagacgttga agctggcgtc tgtgagacct
6901 accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct tgttgaccag ctcggcggtg
6961 acctgcacgt ctagggcgca gtagtccagg gtttccttga tgatgtcata cttatcctgt
7021 cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt ccagtactct
7081 tggatcggaa acccgtcggc ctccgaacgg taagagccta gcatgtagaa ctggttgacg
7141 gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt atgcctgcgc ggccttccgg
7201 agcgaggtgt gggtgagcgc aaaggtgtcc ctgaccatga ctttgaggta ctggtatttg
7261 aagtcagtgt cgtcgcatcc gccctgctcc cagagcaaaa agtccgtgcg ctttttggaa
7321 cgcggatttg gcagggcgaa ggtgacatcg ttgaagagta tctttcccgc gcgaggcata
7381 aagttgcgtg tgatgcggaa gggtcccggc acctcggaac ggttgttaat tacctgggcg
7441 gcgagcacga tctcgtcaaa gccgttgatg ttgtggccca caatgtaaag ttccaagaag
7501 cgcgggatgc ccttgatgga aggcaatttt ttaagttcct cgtaggtgag ctcttcaggg
7561 gagctgagcc cgtgctctga aagggcccag tctgcaagat gagggttgga agcgacgaat
7621 gagctccaca ggtcacgggc cattagcatt tgcaggtggt cgcgaaaggt cctaaactgg
7681 cgacctatgg ccattttttc tggggtgatg cagtagaagg taagcgggtc ttgttcccag
7741 cggtcccatc caaggttcgc ggctaggtct cgcgcggcag tcactagagg ctcatctccg
7801 ccgaacttca tgaccagcat gaagggcacg agctgcttcc caaaggcccc catccaagta
7861 taggtctcta catcgtaggt gacaaagaga cgctcggtgc gaggatgcga gccgatcggg
7921 aagaactgga tctcccgcca ccaattggag gagtggctat tgatgtggtg aaagtagaag
7981 tccctgcgac gggccgaaca ctcgtgctgg cttttgtaaa aacgtgcgca gtactggcag
8041 cggtgcacgg gctgtacatc ctgcacgagg ttgacctgac gaccgcgcac aaggaagcag
8101 agtgggaatt tgagcccctc gcctggcggg tttggctggt ggtcttctac ttcggctgct
8161 tgtccttgac cgtctggctg ctcgagggga gttacggtgg atcggaccac cacgccgcgc
8221 gagcccaaag tccagatgtc cgcgcgcggc ggtcggagct tgatgacaac atcgcgcaga
8281 tgggagctgt ccatggtctg gagctcccgc ggcgtcaggt caggcgggag ctcctgcagg
8341 tttacctcgc atagacgggt cagggcgcgg gctagatcca ggtgatacct aatttccagg
8401 ggctggttgg tggcggcgtc gatggcttgc aagaggccgc atccccgcgg cgcgactacg
8461 gtaccgcgcg gcgggcggtg ggccgcgggg gtgtccttgg atgatgcatc taaaagcggt
8521 gacgcgggcg agcccccgga ggtagggggg gctccggacc cgccgggaga gggggcaggg
8581 gcacgtcggc gccgcgcgcg ggcaggagct ggtgctgcgc gcgtaggttg ctggcgaacg
8641 cgacgacgcg gcggttgatc tcctgaatct ggcgcctctg cgtgaagacg acgggcccgg
8701 tgagcttgaa cctgaaagag agttcgacag aatcaatttc ggtgtcgttg acggcggcct
8761 ggcgcaaaat ctcctgcacg tctcctgagt tgtcttgata ggcgatctcg gccatgaact
8821 gctcgatctc ttcctcctgg agatctccgc gtccggctcg ctccacggtg gcggcgaggt
8881 cgttggaaat gcgggccatg agctgcgaga aggcgttgag gcctccctcg ttccagacgc
8941 ggctgtagac cacgccccct tcggcatcgc gggcgcgcat gaccacctgc gcgagattga
9001 gctccacgtg ccgggcgaag acggcgtagt ttcgcaggcg ctgaaagagg tagttgaggg
9061 tggtggcggt gtgttctgcc acgaagaagt acataaccca gcgtcgcaac gtggattcgt
9121 tgatatcccc caaggcctca aggcgctcca tggcctcgta gaagtccacg gcgaagttga
9181 aaaactggga gttgcgcgcc gacacggtta actcctcctc cagaagacgg atgagctcgg
9241 cgacagtgtc gcgcacctcg cgctcaaagg ctacaggggc ctcttcttct tcttcaatct
9301 cctcttccat aagggcctcc ccttcttctt cttctggcgg cggtggggga ggggggacac
9361 ggcggcgacg acggcgcacc gggaggcggt cgacaaagcg ctcgatcatc tccccgcggc
9421 gacggcgcat ggtctcggtg acggcgcggc cgttctcgcg ggggcgcagt tggaagacgc
9481 cgcccgtcat gtcccggtta tgggttggcg gggggctgcc atgcggcagg gatacggcgc
9541 taacgatgca tctcaacaat tgttgtgtag gtactccgcc gccgagggac ctgagcgagt
9601 ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc taaccagtca cagtcgcaag
9661 gtaggctgag caccgtggcg ggcggcagcg ggcggcggtc ggggttgttt ctggcggagg
9721 tgctgctgat gatgtaatta aagtaggcgg tcttgagacg gcggatggtc gacagaagca
9781 ccatgtcctt gggtccggcc tgctgaatgc gcaggcggtc ggccatgccc caggcttcgt
9841 tttgacatcg gcgcaggtct ttgtagtagt cttgcatgag cctttctacc ggcacttctt
9901 cttctccttc ctcttgtcct gcatctcttg catctatcgc tgcggcggcg gcggagtttg
9961 gccgtaggtg gcgccctctt cctcccatgc gtgtgacccc gaagcccctc atcggctgaa
10021 gcagggctag gtcggcgaca acgcgctcgg ctaatatggc ctgctgcacc tgcgtgaggg
10081 tagactggaa gtcatccatg tccacaaagc ggtggtatgc gcccgtgttg atggtgtaag
10141 tgcagttggc cataacggac cagttaacgg tctggtgacc cggctgcgag agctcggtgt
10201 acctgagacg cgagtaagcc ctcgagtcaa atacgtagtc gttgcaagtc cgcaccaggt
10261 actggtatcc caccaaaaag tgcggcggcg gctggcggta gaggggccag cgtagggtgg
10321 ccggggctcc gggggcgaga tcttccaaca taaggcgatg atatccgtag atgtacctgg
10381 acatccaggt gatgccggcg gcggtggtgg aggcgcgcgg aaagtcgcgg acgcggttcc
10441 agatgttgcg cagcggcaaa aagtgctcca tggtcgggac gctctggccg gtcaggcgcg
10501 cgcaatcgtt gacgctctag cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg
10561 tctggtggat aaattcgcaa gggtatcatg gcggacgacc ggggttcgag ccccgtatcc
10621 ggccgtccgc cgtgatccat gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc
10681 agacaacggg ggagtgctcc ttttggcttc cttccaggcg cggcggctgc tgcgctagct
10741 tttttggcca ctggccgcgc gcagcgtaag cggttaggct ggaaagcgaa agcattaagt
10801 ggctcgctcc ctgtagccgg agggttattt tccaagggtt gagtcgcggg acccccggtt
10861 cgagtctcgg accggccgga ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc
10921 ccgcttgcaa attcctccgg aaacagggac gagccccttt tttgcttttc ccagatgcat
10981 ccggtgctgc ggcagatgcg cccccctcct cagcagcggc aagagcaaga gcagcggcag
11041 acatgcaggg caccctcccc tcctcctacc gcgtcaggag gggcgacatc cgcggttgac
11101 gcggcagcag atggtgatta cgaacccccg cggcgccggg cccggcacta cctggacttg
11161 gaggagggcg agggcctggc gcggctagga gcgccctctc ctgagcggca cccaagggtg
11221 cagctgaagc gtgatacgcg tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc
11281 gagggagagg agcccgagga gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg
11341 catggcctga atcgcgagcg gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc
11401 gggattagtc ccgcgcgcgc acacgtggcg gccgccgacc tggtaaccgc atacgagcag
11461 acggtgaacc aggagattaa ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg
11521 gcgcgcgagg aggtggctat aggactgatg catctgtggg actttgtaag cgcgctggag
11581 caaaacccaa atagcaagcc gctcatggcg cagctgttcc ttatagtgca gcacagcagg
11641 gacaacgagg cattcaggga tgcgctgcta aacatagtag agcccgaggg ccgctggctg
11701 ctcgatttga taaacatcct gcagagcata gtggtgcagg agcgcagctt gagcctggct
11761 gacaaggtgg ccgccatcaa ctattccatg cttagcctgg gcaagtttta cgcccgcaag
11821 atataccata ccccttacgt tcccatagac aaggaggtaa agatcgaggg gttctacatg
11881 cgcatggcgc tgaaggtgct taccttgagc gacgacctgg gcgtttatcg caacgagcgc
11941 atccacaagg ccgtgagcgt gagccggcgg cgcgagctca gcgaccgcga gctgatgcac
12001 agcctgcaaa gggccctggc tggcacgggc agcggcgata gagaggccga gtcctacttt
12061 gacgcgggcg ctgacctgcg ctgggcccca agccgacgcg ccctggaggc agctggggcc
12121 ggacctgggc tggcggtggc acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat
12181 gacgaggacg atgagtacga gccagaggac ggcgagtact aagcggtgat gtttctgatc
12241 agatgatgca agacgcaacg gacccggcgg tgcgggcggc gctgcagagc cagccgtccg
12301 gccttaactc cacggacgac tggcgccagg tcatggaccg catcatgtcg ctgactgcgc
12361 gcaatcctga cgcgttccgg cagcagccgc aggccaaccg gctctccgca attctggaag
12421 cggtggtccc ggcgcgcgca aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc
12481 tggccgaaaa cagggccatc cggcccgacg aggccggcct ggtctacgac gcgctgcttc
12541 agcgcgtggc tcgttacaac agcggcaacg tgcagaccaa cctggaccgg ctggtggggg
12601 atgtgcgcga ggccgtggcg cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca
12661 tggttgcact aaacgccttc ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg
12721 actacaccaa ctttgtgagc gcactgcggc taatggtgac tgagacaccg caaagtgagg
12781 tgtaccagtc tgggccagac tattttttcc agaccagtag acaaggcctg cagaccgtaa
12841 acctgagcca ggctttcaaa aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg
12901 accgcgcgac cgtgtctagc ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag
12961 cgcccttcac ggacagtggc agcgtgtccc gggacacata cctaggtcac ttgctgacac
13021 tgtaccgcga ggccataggt caggcgcatg tggacgagca tactttccag gagattacaa
13081 gtgtcagccg cgcgctgggg caggaggaca cgggcagcct ggaggcaacc ctaaactacc
13141 tgctgaccaa ccggcggcag aagatcccct cgttgcacag tttaaacagc gaggaggagc
13201 gcattttgcg ctacgtgcag cagagcgtga gccttaacct gatgcgcgac ggggtaacgc
13261 ccagcgtggc gctggacatg accgcgcgca acatggaacc gggcatgtat gcctcaaacc
13321 ggccgtttat caaccgccta atggactact tgcatcgcgc ggccgccgtg aaccccgagt
13381 atttcaccaa tgccatcttg aacccgcact ggctaccgcc ccctggtttc tacaccgggg
13441 gattcgaggt gcccgagggt aacgatggat tcctctggga cgacatagac gacagcgtgt
