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平臺編號：bio-114877
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質(zhì)粒信息： pmCherry-Tubulin-C-18
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規(guī)格：2ml甘油管 20ul質(zhì)粒
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用途：/
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
pmCherry-Tubulin-C-18
別稱: Plasmid#55148
復(fù)制子: pUC
質(zhì)粒分類: 基因文庫質(zhì)粒
原核抗性: Kan
篩選標(biāo)記: G418
克隆菌株: DH5a
培養(yǎng)條件: 37度
質(zhì)粒屬性
載體宿主： 哺乳細(xì)胞
載體用途： 蛋白表達(dá)
基因種屬： 人
基因類型： ORF
原核抗性： Kan
真核抗性： Neo/G418
熒光蛋白： 紅色
質(zhì)粒簡介
Homo sapiens tubulin alpha 1b (TUBA1B), mRNA
質(zhì)粒圖譜
質(zhì)粒序列
LOCUS Exported 6072 bp ds-DNA circular SYN 25-FEB-2018
FEATURES Location/Qualifiers
source 1..6072
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 607..616
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 613..1320
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
misc_feature 1376..2730
/label=Tubulin
polyA_signal 2860..2981
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2988..3443)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3470..3574
/gene="bla"
/label=AmpR promoter
promoter 3576..3933
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3784..3919
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3968..4762
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4994..5041
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5370..5958
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
1321 tccggactca gatctggcag cggtggaggc agcgcatccg gcggaagcgg aagcgtgcgt
1381 gagtgcatct ccatccacgt tggccaggct ggtgtccaga ttggcaatgc ctgctgggag
1441 ctctactgcc tggaacacgg catccagccc gatggccaga tgccaagtga caagaccatt
1501 gggggaggag atgactcctt caacaccttc ttcagtgaga cgggcgctgg caagcacgtg
1561 ccccgggctg tgtttgtaga cttggaaccc acagtcattg atgaagttcg cactggcacc
1621 taccgccagc tcttccaccc tgagcagctc atcacaggca aggaagatgc tgccaataac
1681 tatgcccgag ggcactacac cattggcaag gagatcattg accttgtgtt ggaccgaatt
1741 cgcaagctgg ctgaccagtg caccggtctt cagggcttct tggttttcca cagctttggt
1801 gggggaactg gttctgggtt cacctccctg ctcatggaac gtctctcagt tgattatggc
1861 aagaagtcca agctggagtt ctccatttac ccagcacccc aggtttccac agctgtagtt
1921 gagccctaca actccatcct caccacccac accaccctgg agcactctga ttgtgccttc
1981 atggtagaca atgaggccat ctatgacatc tgtcgtagaa acctcgatat cgagcgccca
2041 acctacacta accttaaccg ccttattagc cagattgtgt cctccatcac tgcttccctg
2101 agatttgatg gagccctgaa tgttgacctg acagaattcc agaccaacct ggtgccctac
2161 ccccgcatcc acttccctct ggccacatat gcccctgtca tctctgctga gaaagcctac
2221 catgaacagc tttctgtagc agagatcacc aatgcttgct ttgagccagc caaccagatg
2281 gtgaaatgtg accctcgcca tggtaaatac atggcttgct gcctgttgta ccgtggtgac
2341 gtggttccca aagatgtcaa tgctgccatt gccaccatca aaaccaagcg cagcatccag
2401 tttgtggatt ggtgccccac tggcttcaag gttggcatca actaccagcc tcccactgtg
2461 gtgcctggtg gagacctggc caaggtacag agagctgtgt gcatgctgag caacaccaca
2521 gccattgctg aggcctgggc tcgcctggac cacaagtttg acctgatgta tgccaagcgt
2581 gcctttgttc actggtacgt gggtgagggg atggaggaag gcgagttttc agaggcccgt
2641 gaagatatgg ctgcccttga gaaggattat gaggaggttg gtgtggattc tgttgaagga
2701 gagggtgagg aagaaggaga ggaatactaa ggatccaccg gatctagata actgatcata
2761 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
2821 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
2881 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
2941 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
3001 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
3061 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
3121 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
3181 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
3241 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
3301 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
3361 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
3421 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
3481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
3541 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
3601 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
3661 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
3721 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
3781 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
3841 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
3901 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
3961 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
4021 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
4081 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
4141 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
4201 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
4261 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
4321 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
4381 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
4441 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
4501 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
4561 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
4621 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
4681 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
4741 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
4801 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
4861 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
4921 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
4981 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
5041 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
5101 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
5161 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
5221 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
5281 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
5341 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
5401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
5461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
5521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
5581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
5641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
5701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
5761 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
5821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
5881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
5941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
6001 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
6061 accgccatgc at
