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平臺(tái)編號(hào)：bio-109465
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質(zhì)粒信息： 宿主：DH5α，BL21
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規(guī)格：2ml甘油菌 20ul質(zhì)粒
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用途：酵母系列，酵母單雜交載體，配套pGADT7-rec2,高拷貝載體
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
pHIS2
LB,37℃，氨芐
別稱(chēng):酵母單雜交
啟動(dòng)子:TRP1 ,T7,T3
復(fù)制子:pUC
質(zhì)粒分類(lèi):酵母系列，酵母單雜交載體
質(zhì)粒大小:7207bp
原核抗性:Kan
篩選標(biāo)記:TRP1
克隆菌株:DH5a
培養(yǎng)條件:37度
表達(dá)宿主:酵母細(xì)胞
5'測(cè)序引物:M13F:TGTAAAACGACGGCCAGT
3'測(cè)序引物:根據(jù)序列設(shè)計(jì)引物
質(zhì)粒屬性
載體宿主：酵母菌
載體用途：雜交
基因種屬：
基因類(lèi)型：
原核抗性：Kan
真核抗性：TRP1
熒光蛋白：
質(zhì)粒簡(jiǎn)介
別稱(chēng):酵母單雜交
啟動(dòng)子:TRP1 ,T7,T3
復(fù)制子:pUC
質(zhì)粒分類(lèi):酵母系列，酵母單雜交載體
質(zhì)粒大小:7207bp
原核抗性:Kan
篩選標(biāo)記:TRP1
克隆菌株:DH5a
培養(yǎng)條件:37度
表達(dá)宿主:酵母細(xì)胞
5'測(cè)序引物:M13F:TGTAAAACGACGGCCAGT
3'測(cè)序引物:根據(jù)序列設(shè)計(jì)引物
質(zhì)粒屬性
載體宿主：酵母菌
載體用途：雜交
基因種屬：
基因類(lèi)型：
原核抗性：Kan
真核抗性：TRP1
熒光蛋白：
質(zhì)粒簡(jiǎn)介
pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple cloning site (MCS) and the minimal promoter of the HIS3 locus(PminHIS3). Cis-acting DNA sequences, or DNA target elements, can be inserted into the MCS andused as baits to screen GAL4 AD/cDNA fusion libraries for proteins that interact with the targetsequence. A protein-DNA (or one-hybrid) interaction can be detected by performing the assay in ayeast strain such as Y187 that is auxotrophic for histidine. Positive one-hybrid interactions driveexpression of the HIS3 reporter gene, which enables the host cell to grow on histidine-deficientmedia.
