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平臺(tái)編號(hào)：bio-109460
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質(zhì)粒信息： 宿主：DH5α，BL21
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規(guī)格：2ml甘油菌 20ul質(zhì)粒
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用途：酵母雙雜陰性對(duì)照載體(和pGADT7-T組合形成陰性對(duì)照）
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
pGBKT7-lam
LB,37℃，卡那
啟動(dòng)子:ADH1
復(fù)制子:2U
質(zhì)粒分類:酵母系列，酵母雙雜交載體
質(zhì)粒大小:7.9kb
原核抗性:Kan
篩選標(biāo)記:TRP1
克隆菌株:DH5a
培養(yǎng)條件:37度
表達(dá)宿主:酵母細(xì)胞
5'測序引物:T7:TAATACGACTCACTATAGGG
3'測序引物:根據(jù)序列設(shè)計(jì)引物
質(zhì)粒屬性
載體宿主：酵母菌
載體用途：雜交
基因種屬：
基因類型：
原核抗性：Kan
真核抗性：TRP1
熒光蛋白：
質(zhì)粒簡介
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.
啟動(dòng)子:ADH1
復(fù)制子:2U
質(zhì)粒分類:酵母系列，酵母雙雜交載體
質(zhì)粒大小:7.9kb
原核抗性:Kan
篩選標(biāo)記:TRP1
克隆菌株:DH5a
培養(yǎng)條件:37度
表達(dá)宿主:酵母細(xì)胞
5'測序引物:T7:TAATACGACTCACTATAGGG
3'測序引物:根據(jù)序列設(shè)計(jì)引物
質(zhì)粒屬性
載體宿主：酵母菌
載體用途：雜交
基因種屬：
基因類型：
原核抗性：Kan
真核抗性：TRP1
熒光蛋白：
質(zhì)粒簡介
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.
