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平臺編號：bio-109460
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質(zhì)粒信息： 宿主：DH5α，BL21
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規(guī)格：2ml甘油菌 20ul質(zhì)粒
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用途：酵母雙雜陰性對照載體和pGADT7-T組合形成陰性對照）
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
pGBKT7-lam
| 培養(yǎng)條件 | LB,37℃，卡那 |
| 啟動子 | ADH1 |
| 復(fù)制子 | 2U |
| 質(zhì)粒分類 | 酵母系列，酵母雙雜交載體 |
| 質(zhì)粒大小 | 7.9kb |
| 原核抗性 | Kan |
| 篩選標(biāo)記 | TRP1 |
| 克隆菌株 | DH5a |
| 表達(dá)宿主 | 酵母細(xì)胞 |
| 5'測序引物 | T7:TAATACGACTCACTATAGGG |
| 3'測序引物 | 根據(jù)序列設(shè)計(jì)引物 |
| 載體宿主 | 酵母菌 |
| 載體用途 | 雜交 |
| 原核抗性 | Kan |
| 真核抗性 | TRP1 |
| 質(zhì)粒簡介 | pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced. |
