LB,37℃,氨芐
啟動子:ADH1
復(fù)制子:2U
質(zhì)粒分類:酵母系列,酵母雙雜交載體
質(zhì)粒大小:10.kb
原核抗性:Amp
篩選標(biāo)記:LEU2
克隆菌株:DH5a
培養(yǎng)條件:37度
表達(dá)宿主:酵母細(xì)胞
5'測序引物:T7:TAATACGACTCACTATAGGG
3'測序引物:根據(jù)序列設(shè)計(jì)引物
質(zhì)粒屬性
載體宿主:酵母菌
載體用途:雜交
基因種屬:
基因類型:
原核抗性:Amp
真核抗性:LEU2
熒光蛋白:
質(zhì)粒簡介
pGADT7-T encodes a fusion of the SV40 large T-antigen (a.a. 86–708) and the GAL4 AD (a.a. 768–881). The SV40 large T DNA (GenBank Locus SV4CG) was derived from a plasmid referenced in Li & Fields (1993) and was cloned into pGADT7 using the EcoR I and Xho I sites. pGADT7-T has not been sequenced.