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平臺(tái)編號(hào)：bio-109431
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質(zhì)粒信息： 宿主：DH5α，BL21
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規(guī)格：2ml甘油菌 20ul質(zhì)粒
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用途：哺乳動(dòng)物細(xì)胞質(zhì)粒,Flag標(biāo)簽兩端都有酶切位點(diǎn)的載體
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
p3×Flag-CMV-7.1
基本信息
啟動(dòng)子:CMV復(fù)制子:pUC終止子:SV40 poly(A) signal,hGH poly(A) signal質(zhì)粒分類:哺乳細(xì)胞，蛋白過(guò)表達(dá)載體質(zhì)粒大小:4717bp原核抗性:Amp篩選標(biāo)記:Neo克隆菌株:DH5a培養(yǎng)條件:37度表達(dá)宿主:哺乳細(xì)胞誘導(dǎo)方式:無(wú)須誘導(dǎo)，瞬時(shí)表達(dá)5'測(cè)序引物:CMV-F：CGCAAATGGGCGGTAGGCGTG3'測(cè)序引物:hGH-PA-R:CCAGCTTGGTTCCCAATAGA
質(zhì)粒屬性
載體宿主：哺乳細(xì)胞載體用途：蛋白表達(dá)基因種屬：空載體基因類型：ORF原核抗性：Amp真核抗性：熒光蛋白：
質(zhì)粒簡(jiǎn)介
p3XFLAG-CMV-7.1 Expression Vector is a 4.7 kb derivative of pCMV5 used to establish transient intracellular expression of N-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.
啟動(dòng)子:CMV復(fù)制子:pUC終止子:SV40 poly(A) signal,hGH poly(A) signal質(zhì)粒分類:哺乳細(xì)胞，蛋白過(guò)表達(dá)載體質(zhì)粒大小:4717bp原核抗性:Amp篩選標(biāo)記:Neo克隆菌株:DH5a培養(yǎng)條件:37度表達(dá)宿主:哺乳細(xì)胞誘導(dǎo)方式:無(wú)須誘導(dǎo)，瞬時(shí)表達(dá)5'測(cè)序引物:CMV-F：CGCAAATGGGCGGTAGGCGTG3'測(cè)序引物:hGH-PA-R:CCAGCTTGGTTCCCAATAGA
質(zhì)粒屬性
載體宿主：哺乳細(xì)胞載體用途：蛋白表達(dá)基因種屬：空載體基因類型：ORF原核抗性：Amp真核抗性：熒光蛋白：
質(zhì)粒簡(jiǎn)介
p3XFLAG-CMV-7.1 Expression Vector is a 4.7 kb derivative of pCMV5 used to establish transient intracellular expression of N-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.
