運輸方式：凍存運輸
是否是腫瘤細胞：1
物種來源：人
細胞形態(tài)：混合型
生長狀態(tài)：貼壁生長
數(shù)量：大量
器官來源：其他
年限：64 years
ATCC Number：HTB-118?
相關疾病：腫瘤
規(guī)格：300 ul Designations: SW962 [SW 962, SW-962]
Depositors: ?A Leibovitz
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:mixed
Source: Organ: vulva
Disease: carcinoma
Derived from metastatic site: lymph node
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:Blood type A; Rh+
Cytogenetic Analysis:hypertriploid; modal number = 80; range = 70 to 85. The rate of higher ploidies was 4.3%., At least 15 marker chromosomes were common to most cells. These include: t(1p3p), t(1p17p), i(3q), t(8q9q), del(3)(p21), 20q+ and nine others. Of these, i(3q), t(8q9q), del(3)(p21) and a few others were generally paired., An additional segment on 20q+ is long, but its origin is unknown. The total length of 20q+ is as long as that of N1. Normal N3 was absent, and N1 and N18 had only a single copy per cell. Usually, there were three copies of normal X chromosomes.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1-2
PGM3, 1
Age: 64 years
Gender: female
Ethnicity: Caucasian
Comments:The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian.
A frozen ampule of the line at passage 14 was transferred to the ATCC in January, 1982.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
