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ATCC Number：CRL-5938?
相關(guān)疾病：其他疾病
生長狀態(tài)：貼壁生長
細(xì)胞形態(tài)：球形
是否是腫瘤細(xì)胞：1
物種來源：人
年限：stage 1
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
器官來源：肺
規(guī)格：0.1ml Designations: NCI-H2286 [H2286]
Depositors: ?AF Gazdar, JD Minna
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:loosely adherent
Organism: Homo sapiens
Morphology:rounded


Source: Organ: lung
Tumor Stage: stage 1
Disease: mixed; small cell lung cancer; adenocarcinoma; squamous cell carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
Age: 57 years
Gender: female
Ethnicity: Caucasian
Comments:The line was established in January 1990.
The patient was a smoker.
60 pack years.
Propagation: ATCC complete growth medium: ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:

• 0.02 mg/ml insulin
• 0.01 mg/ml transferrin
• 25 nM sodium selenite (final conc.)
• 50 nM Hydrocortisone (final conc.)
• 1 ng/ml Epidermal Growth Factor (do not filter)
• 0.01 mM ethanolamine (final conc.)
• 0.01 mM phosphorylethanolamine (final conc.)
• 100 pM triiodothyronine (final conc.)
• 0.5% (w/v) bovine serum albumin (final conc.)
• 0.5 mM sodium pyruvate (final conc.)
• extra 2mM L-glutamine (for final conc. of 4.5mM)

Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C

Subculturing: Protocol:

• Remove with floating cells and transfer to a centrifuge tube.
• Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution.
• Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
  Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
• Add 6.0 to 8.0 ml of RPMI 1640 medium supplemented with 10% fetal bovine serum, aspirate cells by gently pipetting and transfer to a centrifuge tube. Pool with cells harvested at step 1. Spin at 125 x g for 5 to 10 minutes. Discard supernatant.
• Resuspend the pellet in ACL-4 medium and dispense into new flasks.
• Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Add fresh medium twice weekly
Preservation: Freeze medium : RPMI 1640 medium, 85%; fetal bovine serum, 10%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..