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平臺編號：bio-69380
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國際編號：HTB-67?
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細(xì)胞信息： SK-MEL-1
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
SK-MEL-1
是否是腫瘤細(xì)胞：1
物種來源：人
器官來源：皮膚
生長狀態(tài)：懸浮生長
ATCC Number：HTB-67?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。簮盒院谏亓?br /> 細(xì)胞形態(tài)：其他
年限：29 years
數(shù)量：大量
規(guī)格：10次 Designations: SK-MEL-1
Depositors: ?G Trempe, LJ Old
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:spherical
Source: Organ: skin
Disease: malignant melanoma
Derived from metastatic site: lymphatic system
Cellular Products:melanin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Applications:Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.
Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Tumorigenic:Yes
Antigen Expression:Blood Type A; Rh+
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 12
THO1: 6
TPOX: 11
vWA: 16,17
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1
Age: 29 years
Gender: male
Ethnicity: Caucasian
Comments:F. Oettgen and associates isolated this line using cells obtained from the thoracic duct of a patient with widespread and rapidly progressing malignant melanoma.
Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.
Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 10 exp5 cells/ml and maintain at 2 X 10 exp5.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 1117: Oettgen HF, et al. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J. Natl. Cancer Inst. 41: 827-843, 1968. PubMed: 4879578
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22816: Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447
23256: Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619
32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241
物種來源：人
器官來源：皮膚
生長狀態(tài)：懸浮生長
ATCC Number：HTB-67?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。簮盒院谏亓?br /> 細(xì)胞形態(tài)：其他
年限：29 years
數(shù)量：大量
規(guī)格：10次 Designations: SK-MEL-1
Depositors: ?G Trempe, LJ Old
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:spherical
Source: Organ: skin
Disease: malignant melanoma
Derived from metastatic site: lymphatic system
Cellular Products:melanin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Applications:Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.
Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Tumorigenic:Yes
Antigen Expression:Blood Type A; Rh+
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 12
THO1: 6
TPOX: 11
vWA: 16,17
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1
Age: 29 years
Gender: male
Ethnicity: Caucasian
Comments:F. Oettgen and associates isolated this line using cells obtained from the thoracic duct of a patient with widespread and rapidly progressing malignant melanoma.
Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis.
Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 10 exp5 cells/ml and maintain at 2 X 10 exp5.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 1117: Oettgen HF, et al. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J. Natl. Cancer Inst. 41: 827-843, 1968. PubMed: 4879578
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22816: Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447
23256: Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619
32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241
