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平臺編號：bio-69081
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國際編號：HB-284?
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細胞信息： 1H3
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
1H3
ATCC Number：HB-284?
是否是腫瘤細胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
數(shù)量：大量
運輸方式：凍存運輸
細胞形態(tài)：淋巴樣
免疫類型：IgG2a
規(guī)格：0.5mg Designations: 1H3
Depositors: ?Mo Dailey, JW Shepherd
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human CD62L
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human peripheral blood leukocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a cell surface molecule (CD62L) that mediates the initial adhesion of lymphocytes and neutrophils to endothelial cells.
The antibody can be used in both immunofluorescence and immunoprecipitation assys.
The cells can be adapted to serum free growth in HB101 medium.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22158: . . FASEB J. 6: 1142, 1992.
是否是腫瘤細胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
數(shù)量：大量
運輸方式：凍存運輸
細胞形態(tài)：淋巴樣
免疫類型：IgG2a
規(guī)格：0.5mg Designations: 1H3
Depositors: ?Mo Dailey, JW Shepherd
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human CD62L
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human peripheral blood leukocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a cell surface molecule (CD62L) that mediates the initial adhesion of lymphocytes and neutrophils to endothelial cells.
The antibody can be used in both immunofluorescence and immunoprecipitation assys.
The cells can be adapted to serum free growth in HB101 medium.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22158: . . FASEB J. 6: 1142, 1992.
