運輸方式：凍存運輸
ATCC Number：HB-191?
生長狀態(tài)：懸浮生長
數(shù)量：大量
是否是腫瘤細胞：0
物種來源：小鼠
細胞形態(tài)：淋巴樣
免疫類型：IgG2a
規(guī)格：0.2ml Designations: PK136
Depositors: ?GC Koo
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse natural killer (NK) cells
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Comments:Animals were immunized with spleen and bone marrow cells from CE mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for NK cells and is cytotoxic in the presence of complement.
The pattern of reactivity of the antibody with cells of various strains of mice matches the pattern of conventional NK 1.1 antisera.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 5 X 10(4) and 1 X 10(6) cells/ml. Do not exceed 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 1319: Koo GC, et al. The NK-1.1 (-) mouse: a model to study differentiation of murine NK cells. J. Immunol. 137: 3742-3747, 1986. PubMed: 3782794
58145: Koo GC, Peppard JR. Establishment of monoclonal anti-NK-1.1 antibody. Hybridoma 3: 301-303, 1984. PubMed: 6500587
