細(xì)胞名稱(chēng):R1/E小鼠胚胎干細(xì)胞R1/E
描述:小鼠全能性胚胎干細(xì)胞,每支細(xì)胞量1×106
動(dòng)物種別:小鼠,129X1 × 129S1品系
性別:雄
組織來(lái)源:小鼠3.5 dpc胚胎內(nèi)細(xì)胞團(tuán)
形態(tài):球形克隆
培養(yǎng)基和添加劑:mES完全培養(yǎng)液配方(600 ml): DMEM (Invitrogen 12430) 497 ml ES級(jí)FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl(終濃度為1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 氣相:空氣,95%;二氧化碳,5%。溫度:37攝氏度。
供應(yīng)限制:A。僅供研究之用。
REFERENCE:Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach. Oxford, Oxford University Press, 101-132, 1999 Nagy A, et al. Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc. Natl. Acad. Sci. USA : 8424-8428, 1993. PubMed: 8378314 Wood SA, et al. Non-injection methods for the production of embryonic stem cell-embryo chimaeras. Nature 365: 87-89, 1993. PubMed: 8361547 Nagy A, Rossant JProduction and analysis of ES-cell aggregation chimeras. In: Nagy A, Rossant J. Gene Targeting: A Practical Approach. Oxford, Oxford University Press, 177-206, 1999
中文名稱(chēng):小鼠胚胎干細(xì)胞R1/E