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產(chǎn)品信息
名稱 DMS 114 (人肺癌細(xì)胞) (STR鑒定正確) 
別稱 DMS-114; DMS114; Darmouth Medical School 114 
種屬 人 
生長(zhǎng)特性 貼壁細(xì)胞 
細(xì)胞形態(tài) 上皮細(xì)胞樣 
生長(zhǎng)培養(yǎng)基 Waymouth's MB 752/1+10%FBS＋1% P/S 
凍存條件 凍存液：55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO溫度：液氮 
培養(yǎng)條件 氣相：空氣，95%；CO2，5%溫度：37℃ 
推薦傳代比例 1:2-1:4 
推薦換液頻率 2-3次/周 
參考資料(來(lái)源文獻(xiàn))
背景描述 The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. 
年齡（性別） 男，68歲 
組織來(lái)源 肺 
細(xì)胞類型 腫瘤細(xì)胞 
腫瘤類型 肺癌細(xì)胞 
生物安全等級(jí) 1 
倍增時(shí)間 3.8 days (PubMed=6266631) 
致瘤性 Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. 
抗原表達(dá)情況 Leu 7 +; My23 +; CD11b + 
基因表達(dá)情況 adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) 
保藏機(jī)構(gòu) ATCC; CRL-2066 
STR鑒定
STR位點(diǎn)信息
Amelogenin X 
CSF1PO 10,11 
D2S1338 17 
D3S1358 14,17 
D5S818 12 
D7S820 10,11 
D8S1179 12,13 
D13S317 13 
D16S539 12 
D18S51 18 
D19S433 13 
D21S11 29 
FGA 21 
PentaD 11,13 
PentaE 7,13 
TH01 8,9.3 
TPOX 8 
vWA 16,17 
D6S1043 12,17 
D12S391 18 
D2S441 10