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Yarrowia lipolytica (Wickerham et al.) van der Walt et von Arx 拉丁名 

(ATCC 20390 ) 統(tǒng)一編號(hào)

Deposited As Yarrowia lipolytica (Wickerham et al.) van der Walt et von Arx, teleomorph 

Strain Designations 別名 D.1805

Synonym Endomycopsis lipolytica Wickerham et al., Saccharomycopsis lipolytica (Wickerham et al.) Yarrow 

Alternate State Candida lipolytica (Harrison) Diddens et Lodder Application Produces citric acid citrate 

Biosafety Level 生物安全等級(jí) 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 freeze-dried 

Storage Conditions 保藏條件

Frozen 冷凍物: -80°C or colder 

Freeze-Dried 凍干物: 2°C to 8°C

Live Culture: See Propagation Section 

Type Strain 模式菌種 no 

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. 

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. 

Preceptrol? no 

Comments 注釋 Self-cycling fermentation 

Morphology After 7 days on YM medium at 25°C, colonies are off white, soft and dull. Slant growth is wrinkled and butyrous. Cells are ovoid to elongate in singles, pairs and clusters. Pseudohyphae are present. 

Medium 培養(yǎng)基 ATCC? Medium 200: YM agar or YM broth 

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 24°C to 26°C 

Atmosphere 需氧情況: Typical aerobic

Sequenced Data D1D2 region of the 26S ribosomal RNA gene GCCTCAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAACCCTCGGGATTGTAATTTGAAGATTTG

GCATTGGAGAAAGCTAACCCAAGTTGCTTGGAATAGTACGTCATAGAGGGTGACAACCCCGTCTGGCTAACCGTTCTCCAT

GTATTGCCTTATCAAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAGTGGGTGGTAAACTCCATCTAAAGCTAAAT

ACTGGTGAGAGACCGATAGCGAACAAGTACTGTGAAGGAAAGGTGAAAAGAACTTTGAAAAGAGAGTGAAATAGTATGTG

AAATTGTTGATAGGGAAGGAAATGAGTGGAGAGTGGCCGAGGTTTCAGCCGCCCCTCGTGGGCGGTGTACTGCCGACGCC

GAGTCATCGATAGCGAGACGAGGGTTACAAATGGGAGCGCCTTTCGGGCGT 

Morphology After 7 days on YM medium at 25°C, colonies are off white, soft and dull. Slant growth is wrinkled and butyrous. Cells are ovoid to elongate in singles, pairs and clusters. Pseudohyphae are present. 

Name of Depositor 寄存者 P Maldonado 

U.S. Patent Number 3,996,106 

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. 

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. 

Chain of Custody 來(lái)源國(guó)家 ATCC <-- P Maldonado <-- C Gaillardin (Endomycopsis lipolytica) 

Isolation 分離基物 Nonsporulating diploid 

References 參考文獻(xiàn) Maldonado P, Charpentier M. Fermentation process for the production of citric and isocitric acids. US Patent 3,997,399 dated Dec 14 1976

Wentworth SD, Cooper DG. Self-cycling fermentation of a citric acid producing strain of Candida lipolytica. J. Ferment. Bioeng. 81: 400-405, 1996. 

French Patent 72/41 913 Briffaud J, Engasser JM. Citric acid production from glucose. I. Growth and excretion kinetics in a stirred fermentor. Biotechnol. Bioeng. 21: 2083-2092, 1979. 

Briffaud J, Engasser JM. Citric acid production from glucose. II. Growth and excretion kinetics in a trickle-flow fermentor. 

Biotechnol. Bioeng. 21: 2093-2111, 1979.