Chlamydia trachomatis Serovar E 拉丁名
(ATCC® VR-348B™) 統(tǒng)一編號(hào)
Classification Chlamydiaceae
Deposited As Chlamydia trachomatis (Busacca) Rake
Agent Chlamydia trachomatis Serovar E Strain BOUR
Applications 用途 Sexually Transmitted Disease Research
Biosafety Level 生物安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions 收藏條件 -70℃ or colder
Comments 注釋 FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. Rapid loss in titer when stored above -70℃. Activities with high potential for aerosol production require BSL 3 facilities and practices.
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies Recommended Host McCoy (ATCC® CRL-1696™)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 35.5℃
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 90-100% confluent. Prior to infection, disrupt cells by sonication or vortex with sterile glass beads. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1). Adsorb by centrifugation at 2100 x g for 1 hour at 20℃. End adsorption by adding growth medium.
Incubation: 3 days
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies
