Aspergillus nidulans (Eidam) Winter
MYA-3632 ™
An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
Product category
Fungi
Strain designation
AN1
Type strain
No
Isolation source
Human biopsy (lung)
Geographical isolation
United States; Maryland; Bethesda
Applications
Agricultural research
Product format
Frozen
Storage conditions
-80°C or colder
General
Specific applications
Biomedical Research and Development Material
Preceptrol
No
Handling information
Medium
ATCC Medium 325: Malt extract agar (Blakeslee's formula)
ATCC Medium 336: Potato dextrose agar (PDA)
ATCC Medium 324: Malt extract agar
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
History
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