Saccharomyces cerevisiae Meyen ex E.C. Hansen 拉丁名
ATCC 201388 編號(hào)
Product category Fungi
Product type Yeast Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain designation 別名 BY4741 [ATCC 4040002]
Type strain 模式菌株 No
Genome sequenced strain Yes
Isolation 分離源 source Derived from S288C, ATCC 204508
Product format 提供形式 Frozen
Storage conditions 保藏溫度 -80°C or colder
Preceptrol No
Mating type a Ploidy Haploid Genotype MATa his3delta1 leu2delta0 met15delta0 ura3delta0 Morphology Cells are globose, ovoidal or elongate, (3.0-8.0) x (5.0-10.0) µm, isolated or in small groups. Sediment is present after one month.
Growth is butyrous and light cream colored. Pseudohyphae are either not formed or are rudimentary.
Comments Parental strain for the International Systematic Saccharomyces cerevisiae Gene Disruption Project
Medium 培養(yǎng)基 ATCC Medium 1245: YEPD
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1069: YPAD medium
Temperature 培養(yǎng)溫度 25-30°C
Atmosphere 需氧情況 Aerobic
Handling procedure Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.
Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).
Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material.
Do not agitate the ampoule. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 µL (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation.
However, the time necessary for significant growth will vary from strain to strain.
Sequenced data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence TGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAATGGATTTTTTTGTTTTGGCAAGAGCATGAGAGCTTTTACTGGGC
AAGAAGACAAGAGATGGAGAGTCCAGCCGGGCCTGCGCTTAAGTGCGCGGTCTTGCTAGGCTTGTAAGTTTCTTTCTTGCTATTCCAAAC
GGTGAGAGATTTCTGTGCTTTTGTTATAGGACAATTAAAACCGTTTCAATACAACACACTGTGGAGTTTTCATATCTTTGCAACTTTTTCTT
TGGGCATTCGAGCAATCGGGGCCCAGAGGTAACAAACACAAACAATTTTATCTATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAAC
TGGAAATTTTAAAATATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAA
TTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCCTTCT
CAAACATTCTGTTTGGTAGTGAGTGATACTCTTTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTCCAAAGAGAGGTT
TCTCTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTTTTTATACTGAGCGTATTGGAACGTT
ATCGATAAGAAGAGAGCGTCTAGGCGAACAATGTTCTTAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAA Verification method
Whole-genome Sequencing
Deposited as Saccharomyces cerevisiae Hansen Synonyms Saccharomyces anamensisWill et Heinrich; Saccharomyces hienipiensis Santa Maria; Saccharomyces steineri var. hara; Saccharomyces batatae Saito; Saccharomyces aceti Santa Maria; Saccharomyces capensis van der Walt et Tscheuschner; Saccharomyces chevalieri Guilliermond; Saccharomyces gaditensis Santa Maria; Saccharomyces cordubensisSanta Maria;Saccharomyces italicus Castelli Depositors JD Boeke Special collection NCRR Contract
Cross references GenBank KU729055 ITS including 5.8S rRNA gene
