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　　Staphylococcus aureus subsp.aureus Rosenbach(ATCC?BAA-44?)

　　Deposited As Staphylococcus aureus Rosenbach

　　Verified By Whole-genome Sequencing

　　Strain Designations HPV107

　　Application

　　Emerging infectious disease research

　　Drug discovery

　　Isolation

　　Iberian clone of MRSA isolated from a hospital in Lisbon,Portugal.

　　Biosafety Level 2

　　Biosafety classification is based on U.S.Public Health Service Guidelines,it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

　　Product Format freeze-dried

　　Storage Conditions Frozen:-80°C or colder

　　Freeze-Dried:2°C to 8°C

　　Live Culture:See Propagation Section

　　Preceptrol?no

　　Genotype pvl negative

　　Type Strain no

　　Antibiotic Resistance

　　Multidrug resistant strain.

　　Resistant to ampicillin,amoxicillin/clavulanic acid,ciprofloxacin,cephalothin,doxycycline,gentamicin,

　　erythromycin,imipenem,methicillin,penicillin,tetracycline,oxacillin,azithromycin,clindamycin,ceftriaxone,rifampin,amikacin and tobramycin.

　　Susceptible to teicoplanin,chloramphenicol,fosfomycin,mupirocin,streptomycin,trimethoprim/sulfamethoxazole,and vancomycin.

　　Intermediate resistance to minocycline.

　　As the guidelines provided by Clinical and Laboratory Standards Institute(CLSI)state,resistance to oxacillin implies resistance to other penicillins such as methicillin.

　　Comments

　　Iberian clone of MRSA

　　SCCmec:Type I

　　PFGE type:Iberian

　　spa type Ridom:t051

　　spa type Kreiswirth:YHFGFMBQBLO

　　pvl gene amplification:Negative

　　Phenotypic and genotypic information for ATCC MRSA strains,such as SCCmec,PFGE,or spa typing,is provided for informational purposes only.This information was compiled from testing performed by one or more sources,including ATCC,strain depositor,published data,or contracted research.

　　Medium ATCC?Medium 18:Trypticase Soy Agar/Broth

　　ATCC?Medium 260:Trypticase soy agar/broth with defibrinated sheep blood

　　Growth Conditions

　　Temperature:37°C

　　Atmosphere:Aerobic

　　Genotype pvl negative

　　Name of Depositor H De Lencastre

　　Special Collection NCRR Contract

　　Isolation

　　Iberian clone of MRSA isolated from a hospital in Lisbon,Portugal.

　　References

　　Monecke S,et al.A field guide to pandemic,epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus.PLoS One 6(4):e17936,2011.PubMed:21494333

　　Mato R,et al.Spread of the multiresistant Iberian clone of methicillin-resistant Staphylococcus aureus(MRSA)to Italy and Scotland.Microb.Drug Resist.4:107-112,1998.PubMed:9650996

　　Sanches IS,et al.Evidence for the geographic spread of a methicillin-resistant Staphylococcus aureus clone between Portugal and Spain.J.Clin.Microbiol.33:1243-1246,1995.PubMed:7615735