Species:Escherichia coli
Group:K12
Strain designation:WG1260
Accession number:LMBP 12432
Depositor:This host strain was deposited by Dr D.E.Culham1 and Prof.Em.Dr J.M.Wood1.
1 Department of Molecular and Cellular Biology,University of Guelph,Guelph,Canada
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:-
Temperature:37°C
Genotype:F-trp lacZ rpsL thi?putPA101?(proU)600?(proP-melAB)212 clsA::Tn10dTet3 proP678::Lumio
Phenotype:StrR TetR
Properties:-
Further information:The allele cls::Tn10dTet3 was introduced into WG1259 by P1 transduction.For the construction of WG1259 the proP678::Lumio ORF was introduced into the chromosome of WG1224 by allelic exchange as described by Link et al.(1997).
Resistance to streptomycin and tetracycline has been experimentally confirmed by BCCM/GeneCorner.
Original reference:Romantsov et al.,J.Bacteriol.192(2010),912-924[PMID:20008071][DOI:10.1128/JB.00967-09]
Related reference:Link et al.,J.Bacteriol.179(1997),6228-6237[PMID:9335267][DOI:10.1128/jb.179.20.6228-6237.1997]
Restricted use:-BCCM MTA
-The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Refer in your Materials and Methods:
Escherichia coli K12 WG1260(LMBP 12432)is available at BCCM/GeneCorner.It was deposited by This host strain was deposited by Dr D.E.Culham and Prof.Em.Dr J.M.Wood and was published in Romantsov et al.,2010.
