基本信息
啟動子: P43
質(zhì)粒分類: 廣宿主系列，枯草桿菌載體
質(zhì)粒大小: 3772bp
原核抗性: Kan
克隆菌株: DH5α
培養(yǎng)條件: 37℃，有氧 LB
表達宿主: 枯草芽孢桿菌
誘導方式: IPTG誘導
5'測序引物: 根據(jù)序列設計引物
3'測序引物: 根據(jù)序列設計引物
質(zhì)粒簡介
pWB705 andpWB980 . pUB110 is the parental plasmid for pUB18 and its derivatives.pWB705 is a pUB18-based secretion vector carrying both the constitutive expressed P43 promoter and the engineered levansucrase signal sequence. pWB980 is a pUB19-based secretion vector. The transcription direction of the P43 promoter is the same as that for the majority of the pUB110-encoded genes. rep, kan, ble, mob and sacB SP represent thesequence coding for replicase, kanamycin resistance marker,bleomycin resistance marker, mobilization protein and the levansucrase signal sequence, respectively. The arrows show the transcription directions for these genes.
